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Recombinant Human CD117 (c-Kit)-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
Mast/stem cell growth factor receptor Kit (SCFR), Piebald traitprotein (PBT), Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 cHardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117
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Product Citations
publications
a. Recom_Human-CD117-Fc-Chimera_1_CF_082724
Recombinant human CD117 (c-Kit)-Fc Chimera binds to human SCF in a dose-dependent manner. EC50 of this effect is 3 - 15 ng/mL.
  • a. Recom_Human-CD117-Fc-Chimera_1_CF_082724
    Recombinant human CD117 (c-Kit)-Fc Chimera binds to human SCF in a dose-dependent manner. EC50 of this effect is 3 - 15 ng/mL.
  • b. Recom_Human-CD117-Fc-Chimera_2_CF_082724
    Stability testing for recombinant human CD117 (c-Kit)-Fc Chimera. Recombinant human CD117-Fc Chimera was aliquoted in PBS, pH 7.2, at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). After this procedure, the samples were tested for their ability to bind to human SCF in a dose-dependent manner. The EC50 for this effect is 3-15 ng/mL.
  • c. Recom_Human-CD117-Fc-Chimera_3_CF_082724
    Recombinant human CD117 (c-Kit)-Fc Chimera inhibits SCF induced cell growth of TF1 cells in a dose-dependent manner.
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597404 25 µg 280€
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597406 100 µg 496€
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Description

The proto-oncogene c-Kit, also known as CD117, was initially identified as a viral oncogene in the Hardy-Zuckerman IV feline sarcoma virus (HZ4- FeSV) and is associated with the transforming activity of the virus. CD117 encodes for a type III receptor tyrosine kinase (RTK), a class of membrane receptors playing a crucial role in cell proliferation, differentiation, and migration through different signaling pathways. Signaling of c-Kit is activated by the binding of its ligand, the stem cell factor (SCF) protein, causing c-Kit dimerization and activation by autophosphorylation and initiating a phosphorylation cascade resulting in the regulation of cell growth. Several studies have highlighted the major role of the SCF and cKit in melanogenesis, gametogenesis, hematopoiesis, and brain angiogenesis. Mutations of KIT frequently occurs in several cancer types and neoplasms including melanoma, gastrointestinal stromal tumors (GISTs), testicular seminomas, mastocytosis and acute myeloid leukaemia (AML). It is suggested that c-Kit acts both as a proto-oncogene via its kinase activity and as a tumor suppressor via its dependence receptor activity. CD117 (c-kit) has been proposed also as a Cancer Stem Cell (CSC) marker in various tumor types. In addition, it is shown that endometrial CSCs expressing CD117 have increased proliferative and colony-forming potential. Not only restricted to endometrial cancer, the expression of CD117 in CSCs has also been demonstrated in ovarian and lung cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Human CD117 (c-Kit), amino acids Gln26-Thr520 (Accession # P10721), with a C-terminal hIgG1-Fc tag, was expressed in 293E cells.
Molecular Mass
The 734 amino acid recombinant protein has a predicted molecular mass of approximately 82.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 110-120 kD and 230-240 kD respectively in SDS-PAGE.
N-terminal
Sequence Analysis
The predicted N-terminal amino acid is Gln.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, pH 7.2
Endotoxin Level
Less than 0.1 EU per μg of cytokine as determined by the LAL method
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human CD117 (c-Kit)-Fc Chimera binds to human SCF in a dose-dependent manner. EC50 of this effect is 3 - 15 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].

Antigen Details

Structure
Monomer
Distribution

Upregulated in several cancer types and neoplasms including melanoma, gastrointestinal stromal tumors (GISTs), testicular seminomas, mastocytosis and acute myeloid leukaemia (AML)

Function
Cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis and mast cell development
Interaction
The stem cell factor (SCF) expressing cells, mostly tumor cells and Cancer Stem Cells (CSCs)
Ligand/Receptor
The stem cell factor (SCF) expressing cells, mostly tumor cells and Cancer Stem Cells (CSCs)
Bioactivity
Recombinant human CD117 (c-Kit)-Fc Chimera binds to human SCF in a dose-dependent manner. EC50 of this effect is 3 - 15 ng/mL.
Cell Type
Hematopoietic stem and progenitors, Leukemia
Biology Area
Angiogenesis, Cancer Biomarkers, Cell Biology, Signal Transduction, Stem Cells
Molecular Family
CD Molecules, Protein Kinases/Phosphatase
Antigen References
  1. Besmer P, et. al. 1986. Nature. 320:415-21.
  2. Ali S. and Ali S. 2007. Gene. 401:38-45.
  3. Zsebo K.M, et. al. 1990. Cell. 63:213-24.
  4. Sattler M and Salgia R. 2004. Leuk. Res. 28:S11-20.
  5. Lennartsson, et. al. 2005. Stem Cells. 23:16-43.
  6. Sun L, et. al. 2006. Cancer Cell. 9:287-300.
  7. Wang H, et. al. 2018. Molecular Cell. 72:413-425.e5.
  8. Beadling C, et. al. 2008. Clin Cancer Res. 14:6821-8
  9. Xu Z, et. al. 2014. Sci. Rep. 4:5907.
  10. Kemmer K, et. al. 2004. Am J Pathol. 164:305-13.
  11. Longley, et. al. 1999. PNAS. 96:1609.
  12. Boissel N, et. al. 2006. Leukemia. 20:965-70.
  13. Giannone, et. al. 2019. Cancers. 11:1820.
  14. Therachiyil L, et. al. 2022. Semin. Cancer Biol. 86:1186-1202.
  15. Zhang X, et. al. 2014. Cancer Lett. 345:106-14
  16. Zhang S, et. al. 2008. Cancer Res. 68:4311-20.
  17. Mazzoldi, et. al. 2019. Cell Death Dis. 10:412.
Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 08-09-2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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