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- Clone
- W22023G (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD137L, 4-1BB-L, 4-1BBL, 41BBL, 4-1BB Ligand, 41BB Ligand
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) (black circles) binds to immobilized recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006). Ultra-LEAF™ purified anti-human CD137L (4-1BB Ligand) (clone W22023G) (purple squares) inhibits the binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400544) (gray triangles) does not have effect. This antibody blocks the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera to 1 µg/mL immobilized recombinant human TNFSF9 (4-1BBL). ND50 range: 0.01 - 0.07 µg/mL.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 647153 | 100 µg | 364€ | ||||
| 647154 | 1 mg | 924€ | ||||
TNFSF9, also known as 4-1BBL and CD137L, is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC), and on B lymphocytes particularly. The interaction between TNFSF9 and its receptor, CD137, which is mainly expressed on T cells, leads to the activation of cytotoxic T cells. In addition, TNFSF9 (CD137) interaction can reactivate anergic T lymphocytes and induce T cell proliferation. This molecule plays a role in optimal CD8 T cell response. Interestingly, it appears that TNFSF9 is able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28. TNFSF9 signaling on T cells has also been shown to limit T cell activation. CD137L is upregulated in peripheral CD14+ monocytes of chronic hepatitis B patients and is associated with liver cirrhosis. Human TNFSF9 shares 36% of its amino acid sequence with its murine counterpart. Mice deficient of TNFSF9 are susceptible to develop B-cell lymphomas.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant Human TNFSF9 (4-1BBL)
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Block - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by blocking the binding of 0.025 µg/mL biotinylated recombinant human TNFRSF9 (4-1BB)-Fc chimera (Cat. No. 772614) to immobilized 1 µg/mL recombinant human TNFSF9 (4-1BBL) (Cat. No. 750006). ND50 range: 0.01 - 0.07 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Antigen Details
- Structure
- Monomer
- Distribution
-
Constitutively expressed on a fraction of dendritic cells (DCs). Inducible on activated monocytes, macrophages, B cells, a small fraction of T cells, epithelial, and endothelial cells
- Function
- TNFSF9 plays roles in cytotoxic T cell activation, T cell proliferation, and apoptosis
- Interaction
- Activated T cells, natural killer cells (NK), macrophages, and dendritic cells
- Ligand/Receptor
- CD137
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
- Antigen References
-
- Vinay DS and Kwon BS. 1998. Semin. Immunol. 10:481-9.
- Watts TH. 2005. Annu Rev Immunol. 23:23:23-68.
- Pollok KE, et al. 1994. Eur J Immunol. 24:367-74.
- Wang J, et al. 2010. J Immunol. 185:367-74.
- Eun SY, et al. 2014. J Immunol. 194:134.
- Spencer AJ, et al. 2014. PLoS One 9:e105520.
- Locatelli F. 2009. Blood. 114:2208-9.
- Gene ID
- 8744 View all products for this Gene ID
- UniProt
- View information about CD137L on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
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No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
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We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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