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Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER119_Alx488_020808
C57BL/6 mouse bone marrow cells stained with TER119 Alexa Fluor® 488
  • TER119_Alx488_020808
    C57BL/6 mouse bone marrow cells stained with TER119 Alexa Fluor® 488
See Alexa Fluor® 488 spectral data
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116215 100 µg 184€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  2. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  3. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  4. Chen C, et al. 2019. Cell Rep. 29:4200. PubMed
  5. Tojo Y, et al. 2015. Mol Cell Biol. 35: 2658-2672. PubMed
  6. Santos J, et al. 2015. Sci Rep. 5: 16034. PubMed
  7. Kehrer J, et al. 2016. PLoS Pathog. 12: 1005734. PubMed
  8. Kehrer J, Frischknecht F, Mair G 2016. Mol Cell Proteomics. 15: 2852 - 2862. PubMed
  9. Meissburger B, et al. 2016. Biochim Biophys Acta. 1861: 1121-1131. PubMed
  10. Yip HYK, et al. 2020. Molecular Cell. 80(2):279-295.e8. PubMed
  11. Yip HYK, et al. 2021. STAR Protocols. 2(3):100765. PubMed
  12. Wang X, et al. 2021. Front Cardiovasc Med. 8:642751. PubMed
  13. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  14. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  15. Zachara M, et al. 2022. EMBO J. 41:e108206. PubMed
RRID
AB_493402 (BioLegend Cat. No. 116215)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org
Go To Top Version: 4    Revision Date: 04-23-2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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