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Alexa Fluor® 700 anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2_Alx700_060308
Human peripheral blood monocytes stained with M5E2 Alexa Fluor® 700
  • M5E2_Alx700_060308
    Human peripheral blood monocytes stained with M5E2 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
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301822 100 µg 215€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Jagannathan P, et al. 2017. Sci Rep. 10.1038/s41598-017-10624-3. PubMed
  2. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  3. Pham TNQ, et al. 2020. Cell Reports. 29(9):2770-2782.e5.. PubMed
  4. Li H, et al. 2015. J Infect Dis. 211:1717. PubMed
  5. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  6. Dudley D, et al. 2016. Nat Commun. 7:12204. PubMed
  7. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  8. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  9. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  10. Sabree SA, et al. 2021. J Immunother Cancer. 9: . PubMed
  11. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  12. O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
  13. Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
  14. Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed
  15. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  16. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  17. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  18. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  19. Connors J, et al. 2022. Geroscience. :. PubMed
  20. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  21. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  22. Castellano E, et al. 2022. Front Immunol. 13:970931. PubMed
RRID
AB_493747 (BioLegend Cat. No. 301822)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 5    Revision Date: 06-27-2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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