Loading...

APC/Cyanine7 anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
HIT3a_APCCy7_122007
Human peripheral blood lymphocytes stained with HIT3a APC/Cyanine7
  • HIT3a_APCCy7_122007
    Human peripheral blood lymphocytes stained with HIT3a APC/Cyanine7
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
300317 25 tests 123€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300318 100 tests 249€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  2. Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
  3. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
  4. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  5. Nakano Y, et al. 2017. PLoS Pathog. 13:e1006348. PubMed
  6. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  7. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  8. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  9. Hinderer S, et al. 2012. Biomaterials. 33:5259. PubMed
  10. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  11. Sato K, et al. 2014. PLoS Pathog. 10:1004453. PubMed
  12. Liu Y, et al. 2015. J Immunol. 194:5851. PubMed
  13. Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed
  14. Bradley S, et al. 2015. PLoS One. 10: 0141171. PubMed
  15. Younis R, et al. 2016. J Immunol. 196: 1419 - 1429. PubMed
  16. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  17. Gerstner S, et al. 2016. J Leukoc Biol. 100(6):1297-1310. PubMed
  18. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  19. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  20. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  21. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  22. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  23. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  24. Kreutmair S, et al. 2021. Immunity. . PubMed
  25. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  26. Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
  27. Harb H, et al. 2020. Nat Immunol. 1359:21. PubMed
  28. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  29. Pache L, et al. 2020. Cell Rep Med. 1:100037. PubMed
  30. Liu R, et al. 2021. Commun Biol. 4:652. PubMed
  31. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  32. Shen J, et al. 2021. Sci Adv. 7:eabi9787. PubMed
  33. Lerrer S, et al. 2021. iScience. 24:103020. PubMed
  34. Singh KS, et al. 2021. Nature. 589:597. PubMed
  35. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  36. Feng Y, et al. 2022. EClinicalMedicine. 43:101226. PubMed
  37. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  38. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  39. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  40. Stunnenberg M, et al. 2021. Eur J Immunol. 51:2464. PubMed
  41. Carrion B, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  42. Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed
  43. Phad GE, et al. 2022. Nat Immunol. 23:1. PubMed
  44. Duque JSR, et al. 2022. Nat Commun. 13:3700. PubMed
  45. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  46. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  47. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  48. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  49. Aoki T, et al. 2020. Cancer Sci. 111:2223. PubMed
  50. Evans WS, et al. 2020. Exp Physiol. 105:1408. PubMed
  51. Sun A, et al. 2021. J Dent Sci. 16:751. PubMed
  52. Yamaguchi A, et al. 2021. J Thorac Dis. 13:5430. PubMed
  53. Chakraborty A, et al. 2022. Methods Mol Biol. 2442:565. PubMed
  54. Schneider MM, et al. 2022. Life Sci Alliance. 5: . PubMed
  55. Stunnenberg M, et al. 2022. J Leukoc Biol. 112:289. PubMed
  56. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  57. Kerns JS, et al. 2023. Bio Protoc. 13: . PubMed
  58. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  59. Tate T, et al. 2023. Cancers (Basel). 15:. PubMed
  60. Tang L, et al. 2023. Front Immunol. 14:1145441. PubMed
RRID
AB_314053 (BioLegend Cat. No. 300317)
AB_314054 (BioLegend Cat. No. 300318)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account