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APC/Fire™ 750 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
M1slash70_APCFire750_CD11b_Antibody_1_032116
C57BL/6 splenocytes were blocked with TruStain fcX™ (anti-mouse CD16/32) Antibody then stained with F4/80 PE and CD11b (clone M1/80) APC/Fire™ 750 (top) or Rat IgG2b, κ APC/Fire™ 750 isotype control (bottom).
  • M1slash70_APCFire750_CD11b_Antibody_1_032116
    C57BL/6 splenocytes were blocked with TruStain fcX™ (anti-mouse CD16/32) Antibody then stained with F4/80 PE and CD11b (clone M1/80) APC/Fire™ 750 (top) or Rat IgG2b, κ APC/Fire™ 750 isotype control (bottom).
  • M1slash70_APCFire750_CD11b_Antibody_2_032116
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability Save
101261 25 µg 90€
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101262 100 µg 212€
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed
  2. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  3. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  4. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  5. Kumar D, et al. 2018. Cancer Prev Res (Phila). 0.895138889. PubMed
  6. Hagan N, et al. 2020. Cell Death Dis. 1.086111111. PubMed
  7. Li Y, et al. 2020. Theranostics. 10:11376. PubMed
  8. C Khouili S, et al. 2020. Cell Rep. 33:108468. PubMed
  9. Barsoumian HB, et al. 2020. J Immunother Cancer. 8:00. PubMed
  10. Ritzel RM, et al. 2021. Glia. 69:746. PubMed
  11. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  12. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  13. Goc J, et al. 2021. Cell. 184:5015. PubMed
  14. Schuran FA, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  15. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  16. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  17. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  18. Sugita Y, et al. 2021. Oncol Rep. 45:. PubMed
  19. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  20. Li Y, et al. 2021. Brain Behav Immun. 91:267. PubMed
  21. Alhudaithi SS, et al. 2020. Mol Pharm. 17:4691. PubMed
  22. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  23. Safaee MM, et al. 2022. Sci Rep. 12:1464. PubMed
  24. Sharma D, et al. 2022. Commun Biol. 5:479. PubMed
  25. Teng F, et al. 2021. Cell Rep. 37:110051. PubMed
  26. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  27. Li Y, et al. 2022. Theranostics. 12:5364. PubMed
  28. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  29. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  30. Tao Z, et al. 2022. Cells. 11:. PubMed
  31. Khan NZ, et al. 2021. Brain Behav Immun. 92:165. PubMed
  32. Veazey JM, et al. 2023. Immunol Cell Biol. 101:130. PubMed
  33. van Elsas MJ, et al. 2023. J Immunother Cancer. 11:. PubMed
  34. Thai LM, et al. 2023. iScience. 26:106477. PubMed
RRID
AB_2572121 (BioLegend Cat. No. 101261)
AB_2572122 (BioLegend Cat. No. 101262)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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