Loading...

Biotin anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RA3-6B2_Biotin_090507
C57BL/6 mouse splenocytes stained with biotinylated RA3-6B2, followed by Sav-PE
  • RA3-6B2_Biotin_090507
    C57BL/6 mouse splenocytes stained with biotinylated RA3-6B2, followed by Sav-PE
  • RA3-6B2_Biotin_B220_Antibody_2_010620
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of biotin anti-mouse B220 (clone RA3-6B2) and Alexa Fluor® 488 anti-mouse CD29 (clone HMβ1-1) (green) overnight at 4°C, followed by 2.5 µg/ml of Spark YG™ 570 Streptavidin (red) for 2 hours at room temperature. The image was captured by 10X objective.
Cat # Size Price Quantity Check Availability Save
103203 50 µg 20€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
103204 500 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Application References
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ishisono K, Yabe T, and Kita K 2017. J Nutr Biochem. . 10.1016/j.jnutbio.2017.07.016. PubMed
  2. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  3. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  4. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  5. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
  6. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  7. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  8. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  9. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  10. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  11. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  12. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  13. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  14. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  15. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  16. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  17. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  18. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  19. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  20. Vural A, et al. 2019. J Immunol. 202:1510. PubMed
  21. Khanom US, et al. 2019. J Immunol. 203:881. PubMed
  22. Leyva‐Castillo JM et al. 2019. Immunity. 50(5):1262-1275 . PubMed
  23. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  24. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  25. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  26. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  27. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  28. Akkaya B, et al. 2017. J Immunol Methods. 441:67. PubMed
  29. Bian Y, et al. 2017. PLoS Pathog. 13:e1006384. PubMed
  30. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  31. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
  32. Thierry GR, et al. 2018. J Exp Med. 215:2972. PubMed
  33. Park DS, et al. 2019. PLoS Comput Biol. 15:e1006913. PubMed
  34. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
  35. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  36. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  37. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  38. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  39. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  40. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  41. Chauveau A, et al. 2020. Immunity. 52:794. PubMed
  42. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  43. Iwata A, et al. 2017. Nat Immunol. 18:563. PubMed
  44. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  45. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  46. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  47. Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
  48. Wang J, et al. 2020. Cell Stem Cell. 27(1):81-97. PubMed
  49. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  50. Shih F, et al. 2006. J Immunol. 176:3438. PubMed
  51. Qin J, et al. 2007. J Immunol. 179:6799. PubMed
  52. Aichinger M, et al. 2013. J Exp Med. 210:287. PubMed
  53. Gally F, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 305:64. PubMed
  54. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  55. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  56. Nishime C, et al. 2015. J Immunol. 195: 1883-1890. PubMed
  57. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  58. Drobek A, et al. 2015. J Immunol. 195: 3416 - 3426. PubMed
  59. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  60. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
  61. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  62. Becklund B, et al. 2016. Sci Rep. 6:30842. PubMed
  63. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  64. Davidson S, et al. 2016. EMBO Mol Med. 8: 1099 - 1112. PubMed
  65. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
  66. Jing Zhou, Jun Jin, Toshihisa Kawai 2016. Sci Rep. 6:39105. PubMed
  67. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  68. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  69. Shen H, et al. 2020. Sci Rep. 10:19453. PubMed
  70. Browning LM, et al. 2020. Cell Rep. 33:108219. PubMed
  71. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  72. Liu YY, et al. 2020. Chin Med J (Engl). 133:1688. PubMed
  73. Matson CA, et al. 2020. Proc Natl Acad Sci U S A. 117:14342. PubMed
  74. Geiger SS, et al. 2021. Nature Communications. 12(1):2745. PubMed
  75. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  76. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
  77. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
  78. Wang F, et al. 2021. Cell. 184(2):422-440.e17. PubMed
  79. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  80. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  81. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  82. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  83. Young K, et al. 2021. Cell Stem Cell. . PubMed
  84. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  85. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  86. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  87. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  88. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  89. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  90. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  91. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
  92. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  93. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  94. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
  95. Rohrbeck L, et al. 2020. Immunol Cell Biol. . PubMed
  96. Lidak T, et al. 2021. Int J Mol Sci. 22: . PubMed
  97. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
  98. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  99. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  100. Dong S, et al. 2021. Nature. 591:117. PubMed
  101. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  102. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  103. Soukupova M, et al. 2021. Mol Ther Methods Clin Dev. 21:399. PubMed
  104. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  105. Chen M, et al. 2021. Cancer Discov. 11:194. PubMed
  106. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  107. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  108. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  109. Haase P, et al. 2020. Eur J Immunol. 50:558. PubMed
  110. Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed
  111. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  112. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  113. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  114. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed
  115. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  116. Zhou L, et al. 2021. Cell Mol Immunol. 18:698. PubMed
  117. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  118. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  119. Guo F, et al. 2022. Front Immunol. 13:833100. PubMed
  120. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  121. D'Agostino MR, et al. 2022. STAR Protoc. 3:101652. PubMed
  122. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  123. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  124. Zhang Y, et al. 2020. J Immunol. 204:3248. PubMed
  125. Zhang MH, et al. 2020. Front Immunol. 11:560. PubMed
  126. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  127. Matsumoto R, et al. 2021. Front Pharmacol. 12:715752. PubMed
  128. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  129. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  130. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  131. Iberg CA, et al. 2022. Cell Rep. 39:110657. PubMed
  132. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  133. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  134. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  135. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  136. del Rio ML, et al. 2022. Front Immunol. 13:887348. PubMed
  137. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  138. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  139. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  140. Tatsumi N, et al. 2022. STAR Protoc. 3:101845. PubMed
  141. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  142. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  143. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  144. Friedman D, et al. 2022. J Immunol. 208:1845. PubMed
  145. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  146. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
RRID
AB_312988 (BioLegend Cat. No. 103203)
AB_312989 (BioLegend Cat. No. 103204)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 3    Revision Date: 01-06-2020

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account