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Brilliant Violet 421™ anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_BV421_1_032911
Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • HIB19_BV421_1_032911
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD19 (clone HIB19) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • HIB19_BV421_2_032911
  • HIB19_BV421_CD19_Antibody_2_012914
    Human peripheral mononuclear cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/mL CD19 (clone HIB19) Brilliant Violet 421™ (blue) and 5 µg/mL CD3 (clone UCHT1) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.
  • HIB19_BV421_CD19_Antibody_3_012914
    Human frozen tonsil tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 5 µg/mL of Alexa Fluor® 488 anti-human CD3 antibody (green) and Brilliant Violet 421™ anti-human CD19 antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
302233 25 tests 152€
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302234 100 tests 305€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
  2. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  3. Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed
  4. Antonarakis ES, et al. 2018. Clin Cancer Res. 24:4662. PubMed
  5. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  6. Schoofs T et al. 2019. Immunity. 50(6):1513-1529 . PubMed
  7. Ju CH, et al. 2018. Clin Immunol. 193:70. PubMed
  8. Shih T, et al. 2019. Front Immunol. 10:1652. PubMed
  9. Zumaquero E, et al. 2019. Elife. 8. PubMed
  10. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  11. Koppejan H, et al. 2019. Arthritis Res Ther. 21:03. PubMed
  12. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  13. Singh M, et al. 2020. Cell. 180(5):878-894. PubMed
  14. Tan Y, et al. 2014. Cell Immunol. 151:55. PubMed
  15. Lu D, et al. 2014. Cell Immunol. 152:77. PubMed
  16. O’Connor K, et al. 2016. Genes Immun. 10.1038/gene.2016.27. PubMed
  17. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed
  18. Amara K, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.03.004. PubMed
  19. Travaglini KJ, et al. 2020. Nature. 587:619. PubMed
  20. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  21. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  22. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  23. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  24. Panwar B, et al. 2021. Genome Res. 31:659. PubMed
  25. Caeser R, et al. 2021. Nat Protoc. 16:2499. PubMed
  26. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  27. Turner JS, et al. 2021. Nature. 596:109. PubMed
  28. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  29. De Silva TI, et al. 2021. iScience. 24:103353. PubMed
  30. NULL, et al. 2020. J Clin Immunol. 40:1. PubMed
  31. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  32. Vijayakumar B, et al. 2022. Immunity. . PubMed
  33. Zheng H, et al. 2022. Cell Rep. 39:110864. PubMed
  34. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  35. Sun Q, et al. 2022. Cell Death Dis. 13:724. PubMed
  36. Turner JS, et al. 2020. Nature. 586:127. PubMed
  37. Mimitou EP, et al. 2021. Nat Biotechnol. 39:1246. PubMed
  38. Tian P, et al. 2022. Front Cell Dev Biol. 10:835321. PubMed
  39. Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
  40. De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed
  41. Beaudoin-Bussières G, et al. 2022. Viruses. 14: . PubMed
  42. Barnes CO, et al. 2022. Sci Adv. 8:eabp8155. PubMed
  43. Asashima H, et al. 2022. J Clin Invest. 132: . PubMed
  44. Ringelstein-Harlev S, et al. 2023. Eur J Haematol. 110:371. PubMed
  45. Chauvin C, et al. 2023. iScience. 26:106124. PubMed
  46. García-Vega M, et al. 2023. iScience. 26:106562. PubMed
  47. Takeshita M, et al. 2023. iScience. 26:106955. PubMed
RRID
AB_10897802 (BioLegend Cat. No. 302233)
AB_11142678 (BioLegend Cat. No. 302234)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 4    Revision Date: 03-19-2020

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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