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Brilliant Violet 570™ anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_BV570_1_072011
Human peripheral blood lymphocytes were stained with CD19 APC and CD3 (clone UCHT1) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
  • UCHT1_BV570_1_072011
    Human peripheral blood lymphocytes were stained with CD19 APC and CD3 (clone UCHT1) Brilliant Violet 570™ (top) or mouse IgG1, κ Brilliant Violet 570™ isotype control (bottom).
  • UCHT1_BV570_2_072011
See Brilliant Violet 570™ spectral data
Cat # Size Price Quantity Check Availability Save
300435 25 tests 168€
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300436 100 tests 348€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  2. Aung Naing et al. 2018. Cancer cell. 34(5):775-791 . PubMed
  3. Meehan S, et al. 2019. Commun Biol. 2:229. PubMed
  4. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  5. Cortés–Rubio CN, et al. 2019. Clin Epigenetics. 11:134. PubMed
  6. Fernandez IZ, et al. 2019. J Exp Med. 216:1255. PubMed
  7. Marquez EJ, et al. 2020. Nat Commun. 11:751. PubMed
  8. Iversen R, et al. 2019. Proc Natl Acad Sci U S A. 116:15134. PubMed
  9. Boer M, et al. 2015. Clin Vaccine Immunol. 22: 778 - 788. PubMed
  10. Di Niro R, et al. 2015. Mucosal Immunol. . PubMed
  11. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
  12. Demers K, et al. 2016. PLoS Pathog. 12: 1005805. PubMed
  13. Briceño O, et al. 2016. PLoS One. 11:e0166496. PubMed
  14. Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed
  15. Nguyen T, et al. 2017. Clin Transl Immunology. 10.1038/cti.2017.4. PubMed
  16. Pinto-Cardoso S, et al. 2017. Sci Rep. 10.1038/srep43741. PubMed
  17. Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed
  18. Pattekar A, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 11(5):1267-1289. PubMed
  19. Carre C, et al. 2021. iScience. 24:102970. PubMed
  20. Cordero H, et al. 2021. Nat Commun. 12:5761. PubMed
  21. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
  22. Kourtis N, et al. 2022. Nat Cancer. :. PubMed
  23. Page DB, et al. 2022. NPJ Breast Cancer. 8:50. PubMed
  24. Hackstein CP, et al. 2022. Nat Commun. 13:7472. PubMed
  25. Xu C, et al. 2022. iScience. 25:105123. PubMed
  26. Buckner CM, et al. 2022. Cell. 185:4333. PubMed
  27. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  28. Hilliard S, et al. 2023. iScience. 26:106041. PubMed
RRID
AB_10898117 (BioLegend Cat. No. 300435)
AB_2562124 (BioLegend Cat. No. 300436)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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