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Brilliant Violet 605™ anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A019D5_BV605_CD127_Antibody_FC_1_043013
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).
  • A019D5_BV605_CD127_Antibody_FC_1_043013
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) Brilliant Violet 605™ (top) or mouse IgG1, κ Brilliant Violet 605™ isotype control (bottom).
  • A019D5_BV605_CD127_Antibody_FC_2_043013
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
351333 25 tests 181€
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351334 100 tests 357€
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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Application References
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
  2. Cella M, et al. 2019. Nat Immunol. 1.513888889. PubMed
  3. Sekine T, et al. 2020. Cell. 183:158. PubMed
  4. Kim P, et al. 2014. J Immunol. 192:2622. PubMed
  5. Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed
  6. Rueschenbaum S, et al. 2021. Front Immunol. 11:581352. PubMed
  7. Wild K, et al. 2021. Nat Commun. 12:6720. PubMed
  8. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  9. Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
  10. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  11. Gao X, et al. 2022. iScience. 25:104911. PubMed
  12. Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
  13. Riese P, et al. 2022. Nat Commun. 13:6894. PubMed
  14. McMurray JL, et al. 2022. Cell Rep. 39:110858. PubMed
  15. Ke F, et al. 2023. Elife. 12:. PubMed
RRID
AB_2562019 (BioLegend Cat. No. 351333)
AB_2562022 (BioLegend Cat. No. 351334)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 05-01-2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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