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FITC anti-human CD206 (MMR) Antibody

Pricing & Availability
Clone
15-2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype
Mouse IgG1, κ
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Product Citations
publications
15-2_FITC_111208.jpg
GM-CSF stimulated (day3) human peripheral blood monocytes stained with 15-2 FITC
  • 15-2_FITC_111208.jpg
    GM-CSF stimulated (day3) human peripheral blood monocytes stained with 15-2 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
321103 25 tests 85€
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321104 100 tests 184€
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Description

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human mannose receptor
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).

Application References
  1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
  2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
  3. Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations
  1. Mifuji K, et al. 2017. Bone Joint Res. 10.1302/2046-3758.68.BJR-2016-0338.R1. PubMed
  2. Shen M, et al. 2018. Int J Oncol. 53:317. PubMed
  3. Krakow S, et al. 2019. Front Immunol. 10:1484. PubMed
  4. Serhan J 2012. Blood. 120:e60. PubMed
  5. Zhang Q, et al. 2013. Clin Cancer Res. 15:3176. PubMed
  6. Herter S, et al. 2013. Mol Cancer Ther. 12:2031. PubMed
  7. Ball M, et al. 2016. PLoS One. 11: 0149600. PubMed
  8. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  9. Dai X, et al. 2021. Bioact Mater. 1.659027778. PubMed
  10. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  11. Aung A, et al. 2020. Cancer Res. 80:263. PubMed
  12. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  13. Winkler C, et al. 2021. JCI Insight. 6:. PubMed
  14. Conforti A, et al. 2021. PLoS One. 16:e0259894. PubMed
  15. Feuerer N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  16. Yoshimori M, et al. 2021. Cancers (Basel). 13:. PubMed
  17. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  18. Teng YY, et al. 2022. Front Bioeng Biotechnol. 10:902894. PubMed
  19. Rodriguez-Perdigon M, et al. 2022. Macromol Biosci. 22:e2200168. PubMed
  20. Wu JJ, et al. 2022. Cell Death Dis. 13:527. PubMed
  21. Nyazika TK, et al. 2022. Front Immunol. 13:992659. PubMed
  22. Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed
  23. Xu G, et al. 2022. Cell Death Dis. 13:888. PubMed
  24. Gelsomino L, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Chung JY, et al. 2023. Nat Commun. 14:1794. PubMed
  26. Chen S, et al. 2023. J Immunother Cancer. 11:. PubMed
  27. Lin H, et al. 2023. J Transl Med. 21:343. PubMed
  28. Rodriguez-Perdigon M, et al. 2023. Front Bioeng Biotechnol. 11:1159819. PubMed
RRID
AB_571904 (BioLegend Cat. No. 321103)
AB_571905 (BioLegend Cat. No. 321104)

Antigen Details

Structure
Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution

Macrophages, dendritic cells, hepatic and lymphatic endothelial cells

Function
Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor
Mannose, fucose, N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.

Gene ID
4360 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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