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FITC anti-human HLA-DR Antibody

Pricing & Availability
Clone
L243 (See other available formats)
Regulatory Status
RUO
Other Names
Major Histocompatibility Class II, MHC class II
Isotype
Mouse IgG2a, κ
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Product Citations
publications
L243_FITC_072607
Human peripheral blood lymphocytes stained with L243 FITC
  • L243_FITC_072607
    Human peripheral blood lymphocytes stained with L243 FITC
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
307603 25 tests 49€
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307604 100 tests 111€
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307632 100 µg 118€
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Description

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

For flow cytometric staining using the µg size, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. For flow cytometric staining using test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The L243 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Clone L243 binds a conformational epitope on HLA-DRa which depends on the correct folding of the aß heterodimer.19

Additional reported applications (for the relevant formats) include: immunoprecipitation8, Western blotting8, in vitro blocking of mixed lymphocyte reactions9,10, depeletion of MHC class II cells7, immunohistochemical staining of acetone-fixed frozen sections4,5, and spatial biology (IBEX)21,22. For sensitive functional assays, we recommend using the Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 307648, 307665 - 307669).

Application References
  1. Brodsky F. 1984. Immunogenetics 19:179.
  2. Robbins P, et al. 1987. Human Immunol. 18:301.
  3. Stites D, et al. 1986. Clin. Immunol. Immunopathol. 38:161.
  4. Warnke R, et al. 1980. J. Histochem. Cytochem. 28:771. (IHC)
  5. Engleman E, et al. 1981. P. Natl. Acad. Sci. USA 78:1791. (IHC)
  6. Zipf T, et al. 1981. Cancer Res. 41:4786.
  7. Goodier M, et al. 2000. J. Immunol. 165:139. (Depletion)
  8. Esser M, et al. 2001. J. Virol. 75:6173. (IP, WB)
  9. Kalka-Moll WM, et al. 2002. J. Immunol. 169:6149. (Block)
  10. Wang RF, et al. 1999. Science 284:1351. (Block)
  11. Zaba LC, et al. 2007. J. Exp. Med. 204:3183. PubMed
  12. Fujita H, et al. 2009. P. Natl. Acad. Sci. USA 106:21795. PubMed
  13. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  14. Goncalves RM, et al. 2010. Infect. Immun. 78:4763. PubMed
  15. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  16. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  17. Stein R, et al. 2011. Leuk. Lymphoma 52:273.
  18. Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
  19. Moro M, et al. 2005. BMC Immunol. 6:24.
  20. Lauterbach N, et al. 2014. Mol Immunol. 59:19. PubMed
  21. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  22. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Neuser J, et al. 2017. PLoS One.. 10.1371/journal.pone.0183670. PubMed
  2. Fang W,et al. 2017. PLoS One. 10.1371/journal.pone.0187088. PubMed
  3. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  4. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  5. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  6. Shan L, et al. 2017. Immunity. 47:766. PubMed
  7. Oshchepkova A, et al. 2019. Micromachines (Basel). 0.9375. PubMed
  8. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  9. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  10. Wang C, et al. 2020. Oncologist. 25:382. PubMed
  11. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  12. Wang X, et al. 2012. J Immunol. 189:3092. PubMed
  13. Iida H, et al. 2008. J Dermatol Sci. 52:31. PubMed
  14. Hsieh J, et al. 2013. Nucleic Acids Res. 41:9753. PubMed
  15. Planès R, et al. 2014. J Virol. 88:6672. PubMed
  16. Chao Y, et al. 2014. J Leukoc Biol. 96:1037. PubMed
  17. Lathrop S, et al. 2015. Infect Immun . 83: 2661 - 2671. PubMed
  18. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  19. Serrano-Villar S, et al. 2016. PLoS Pathog. 12: 1005381. PubMed
  20. Gadd V, et al. 2016. PLoS One. 11: 0157771. PubMed
  21. Muenchhoff M, et al. 2016. Sci Transl Med. 8: 358ra125. PubMed
  22. Santiago K, et al. 2016. Pathog Dis. 74: ftw081. PubMed
  23. Sade-Feldman M, et al. 2016. Clin Cancer Res. 22(23):5661-5672. PubMed
  24. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  25. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  26. Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed
  27. Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed
  28. Keshtkar S, et al. 2021. Stem Cells Int. 8857457:2020. PubMed
  29. Le Voyer T, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  30. Jøntvedt Jørgensen M, et al. 2021. Front Cell Infect Microbiol. 11:669623. PubMed
  31. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  32. Das J, et al. 2021. Med (N Y). 2:1269. PubMed
  33. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  34. Port JR, et al. 2020. J Virol. 94:. PubMed
  35. Li J, et al. 2021. Front Cell Dev Biol. 9:737242. PubMed
  36. Ren K, et al. 2022. Brain Behav. 12:e2456. PubMed
  37. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  38. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  39. Lee PC, et al. 2022. J Clin Invest. 132:. PubMed
  40. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  41. Li J, et al. 2022. Int J Mol Sci. 23:. PubMed
  42. Menges D, et al. 2022. Nat Commun. 13:4855. PubMed
  43. Yan K, et al. 2022. iScience. 25:104822. PubMed
  44. Ran Y, et al. 2022. Stem Cell Res Ther. 13:507. PubMed
  45. Raggi C, et al. 2022. Curr Protoc. 2:e389. PubMed
  46. Scirocchi F, et al. 2022. EBioMedicine. 79:104010. PubMed
  47. Davies LRL, et al. 2022. Sci Transl Med. 14:eabm4065. PubMed
  48. Sharma AL, et al. 2022. iScience. 25:105651. PubMed
  49. Boudreau CM, et al. 2023. Cell Rep Med. 4:100975. PubMed
  50. Guo Q, et al. 2023. Front Immunol. 14:1117545. PubMed
  51. Jiang B, et al. 2023. Sci Signal. 16:eabo3406. PubMed
  52. Ishiuchi N, et al. 2023. Stem Cell Res Ther. 14:121. PubMed
  53. Aabling RR, et al. 2023. Regen Ther. 23:67. PubMed
  54. Smith MH, et al. 2023. Nat Immunol. . PubMed
RRID
AB_314681 (BioLegend Cat. No. 307603)
AB_314682 (BioLegend Cat. No. 307604)
AB_1089142 (BioLegend Cat. No. 307632)

Antigen Details

Structure
Ig superfamily, MHC class II, heterodimeric transmembrane protein, 36 kD heavy and 27 kD light chain
Distribution

B cells, activated T cells, monocytes/macrophages, dendritic cells, other APCs

Function
Peptide presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Levacher M, et al. 1990. Clin. Exp. Immunol. 81:177.
2. Terstappen L, et al. 1990. J. Leukocyte Biol. 48:138.
3. Edwards JA, et al. 1986. J. Immunol. 137:490.
4. van Es A, et al. 1984. Transplantation 37:65.
5. O'Doherty U, et al. 1994. Immunology 82:487.
6. Thomas R, et al. 1994. J. Immunol. 153:4016.
7. Grouard G, et al. 1996. Nature 384:364.

Gene ID
3122 View all products for this Gene ID 3123 View all products for this Gene ID
UniProt
View information about HLA-DR on UniProt.org
Go To Top Version: 3    Revision Date: 06-17-2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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