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PE anti-human CD152 (CTLA-4) Antibody

Pricing & Availability
Clone
L3D10 (See other available formats)
Regulatory Status
RUO
Other Names
Cytotoxic T-Lymphocyte Antigen 4
Isotype
Mouse IgG1, κ
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Product Citations
publications
L3D10_PE_CD152_Antibody_011822
PMA/Ionomycin-stimulated human peripheral blood lymphocytes were stained with anti-human CD4 APC, then fixed, permeabilized and intracellularly stained with anti-CD152 (CTLA-4) (clone L3D10) PE (left) or Mouse IgG1, κ isotype control PE (right).
  • L3D10_PE_CD152_Antibody_011822
    PMA/Ionomycin-stimulated human peripheral blood lymphocytes were stained with anti-human CD4 APC, then fixed, permeabilized and intracellularly stained with anti-CD152 (CTLA-4) (clone L3D10) PE (left) or Mouse IgG1, κ isotype control PE (right).
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349905 25 tests 94€
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349906 100 tests 224€
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Description

CD152, also known as Cytotoxic T-Lymphocyte Antigen 4 (CTLA-4), is a 33 kD member of the immunoglobulin superfamily. It is transiently expressed on activated T cells. CTLA4 is expressed on the surface of helper T cells and transmits an inhibitory signal to T cells. Regulatory T cells express high levels of CTLA-4. CTLA-4 (CD152) is similar to CD28 in amino acid sequence, structure, and genomic organization. Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses through interaction with CD80 (B7-1) and CD86 (B7-2) present on antigen presenting cells (APC). CTLA-4 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Mutations in the CTLA-4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. A transcript of the CTLA-4 gene that may represent a native soluble form of CTLA-4 (sCTLA-4) showed that eleven of twenty patients with autoimmune thyroid disease (ATD) had a high concentration of sCTLA-4, whereas only 1 of 30 apparently healthy volunteers contained measurable levels. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of human CTLA-4 and human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Detection: The biotinylated L3D10 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified A3.6B10.G1 antibody (Cat. No. 525401) as the capture antibody and recombinant human CTLA-4 (Cat. No. 591909) as the standard.

Flow Cytometry: The fluorochrome-labeled L3D10 antibody is useful for immunofluorescent staining and flow cytometric analysis to identify CTLA-4-producing cells within mixed cell populations.

Note: For testing human soluble CTLA-4 in serum, plasma or cell culture supernatant, LEGEND MAX™ Human Soluble CTLA-4 ELISA Kit with Pre-coated Plates (Cat. No. 437407) are specially developed and recommended.

Additional reported applications (for the relevant formats) include: Blocking of CTLA-4/B7-1 interaction and blocking of CTLA-4-mediated inhibitory function to promote T cell expansion1,2.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of frozen or paraffin-embedded tissue sections (IHC-F or IHC-P).

Application References
  1. May K, et al. 2005. Blood 105:1114. (Block)
  2. Lute K, et al. 2005. Blood 106:3127. (Block)
Product Citations
  1. Cao J, et al. 2018. Nat Commun. 9:77. PubMed
  2. Cuadrado E, et al. 2018. Immunity. 48:1046. PubMed
  3. Ickrath P, et al. 2019. Biomed Rep. 10:119. PubMed
  4. Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed
  5. Ickrath P, et al. 2018. Int J Mol Med. 42:1116. PubMed
  6. Cunningham RA, et al. 2019. J Biol Methods. 6:e114. PubMed
  7. Manuszak C, et al. 2020. J Biol Methods. 7:e131. PubMed
  8. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  9. Wei F, et al. 2013. Proc Natl Acad Sci U S A. 110:2480. PubMed
  10. Hatano R, et al. 2015. J Immunol. 194:960. PubMed
  11. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  12. Wang S, et al. 2021. Exp Ther Med. 21:37. PubMed
  13. Bilich T, et al. 2021. Cancer Discov. 11:1982. PubMed
  14. Zhao Y, et al. 2021. Front Immunol. 12:665442. PubMed
  15. Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
  16. Wang X, et al. 2022. J Exp Clin Cancer Res. 41:210. PubMed
  17. Lanna A, et al. 2022. Nat Cell Biol. 24:1461. PubMed
  18. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  19. Cheng K, et al. 2023. Int J Mol Sci. 24:. PubMed
  20. Felce SL, et al. 2021. Wellcome Open Res. 5:42. PubMed
RRID
AB_10645522 (BioLegend Cat. No. 349905)
AB_10641842 (BioLegend Cat. No. 349906)

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells and B cells

Function
Negative regulator of T cell activation
Interaction
Antigen presenting cells, such as dendritic cells
Ligand/Receptor
B7-1 (CD80), B7-2 (CD86)
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay N, et al. The Leukocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Kuiper H, et al. 1995. J. Immunol. 155:1776.
3. Lindsten T, et al. 1993. J. Immunol. 151:3489.
4. Morton P, et al. 1996. J. Immunol. 156:1047.

Gene ID
1493 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07-24-2024

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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