Loading...

PE anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
HIB19_PE_061807
Human peripheral blood lymphocytes stained with HIB19 PE
  • HIB19_PE_061807
    Human peripheral blood lymphocytes stained with HIB19 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
302207 25 tests 23€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
302208 100 tests 53€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
302254 100 µg 59€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  2. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  3. Basnett J, et al. 2017. PLoS One. . 10.1371/journal.pone.0188042. PubMed
  4. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  5. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  6. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  7. Xiao X, et al. 2019. Cell Discov. 5:2. PubMed
  8. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  9. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  10. Cho A, et al. 2019. Cell Rep. 28:909. PubMed
  11. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
  12. Zumaquero E, et al. 2019. Elife. 8. PubMed
  13. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  14. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  15. Luong-Gardiol N, et al. 2019. Cancer Cell. 35:649. PubMed
  16. Jiang Q et al. 2019. Cancer cell. 35(1):81-94 . PubMed
  17. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  18. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  19. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  20. Greiner V, et al. 2019. iScience. 0.75625. PubMed
  21. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Li W, et al. 2020. Immunity. 53(2):456-470. PubMed
  24. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  25. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  26. Huang Y, et al. 2020. Cancer Cell. 36(6):630-644.e9.. PubMed
  27. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  28. Yamanaka M, et al. 2009. Glycobiology. 1.375694444. PubMed
  29. White C, et al. 2014. J Immunol. 193:5933. PubMed
  30. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  31. Matsui K, et al. 2015. PLoS One. 10: 0137195. PubMed
  32. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  33. McComb S, et al. 2016. Sci Transl Med. 8: 339ra70. PubMed
  34. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  35. Kreye J, et al. 2020. Cell. 183(4):1058-1069.e19. PubMed
  36. de Jonge K, et al. 2021. OncoImmunology. 10(1):1873585. PubMed
  37. Frame JM, et al. 2020. Developmental Cell. 55(2):133-149.e6. PubMed
  38. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  39. Mondala PK, et al. 2021. Cell Stem Cell. 28(4):623-636.e9. PubMed
  40. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
  41. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  42. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  43. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  44. Vora AA, et al. 2021. STAR Protocols. 2(2):100565. PubMed
  45. Kreye J, et al. 2020. bioRxiv. . PubMed
  46. Zheng L, et al. 2020. Clin Cancer Res. 26:3694. PubMed
  47. Amanat F, et al. 2021. Cell. 184:3936. PubMed
  48. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  49. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  50. Madsen A, et al. 2020. Immunity. 53:852. PubMed
  51. Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed
  52. Zhang Q, et al. 2021. Bone Joint Res. 10:226. PubMed
  53. Malik-Chaudhry HK, et al. 2021. MAbs. 13:1890411. PubMed
  54. Caeser R, et al. 2021. Nat Protoc. 16:2499. PubMed
  55. Han AR, et al. 2021. Int J Stem Cells. 14:203. PubMed
  56. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  57. Shen J, et al. 2021. Sci Adv. 7:eabi9787. PubMed
  58. Jing Y, et al. 2021. Signal Transduct Target Ther. 6:345. PubMed
  59. Roider T, et al. 2021. Blood Adv. 5:5060. PubMed
  60. Wang L, et al. 2021. Microbiol Spectr. 9:e0124621. PubMed
  61. Turner JS, et al. 2021. Nature. 595:421. PubMed
  62. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  63. Wang X, et al. 2021. Front Endocrinol (Lausanne). 12:666195. PubMed
  64. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  65. Ruan Y, et al. 2022. Exp Ther Med. 23:47. PubMed
  66. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  67. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  68. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  69. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  70. Schlicher L, et al. 2022. Int J Mol Sci. 23:. PubMed
  71. Cai S, et al. 2022. Cell Biosci. 12:115. PubMed
  72. Gu X, et al. 2022. Vaccines (Basel). 10:. PubMed
  73. Sipe CJ, et al. 2022. Int J Mol Sci. 23:. PubMed
  74. SoRelle ED, et al. 2022. Cell Rep. 40:111286. PubMed
  75. Turner JS, et al. 2020. Nature. 586:127. PubMed
  76. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  77. Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed
  78. Fisher JG, et al. 2021. Front Oncol. 11:785635. PubMed
  79. Kim ST, et al. 2022. Nat Commun. 13:1970. PubMed
  80. Moquin-Beaudry G, et al. 2022. Cell Rep Methods. 2:100153. PubMed
  81. Avsec D, et al. 2022. Cell Death Dis. 13:860. PubMed
  82. SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed
  83. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  84. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  85. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  86. Polisetti N, et al. 2022. Bio Protoc. 12: . PubMed
  87. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  88. Tolcher AW, et al. 2023. J Immunother Cancer. 11: . PubMed
  89. Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed
  90. Im NG, et al. 2022. Cancer Immunol Res. 10:1055. PubMed
  91. Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed
  92. Smith MH, et al. 2023. Nat Immunol. . PubMed
RRID
AB_314237 (BioLegend Cat. No. 302207)
AB_314238 (BioLegend Cat. No. 302208)
AB_2564142 (BioLegend Cat. No. 302254)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12-18-2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account