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PE anti-human CD33 Antibody

Pricing & Availability
Clone
WM53 (See other available formats)
Regulatory Status
RUO
Workshop
IV M-505
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM53_PE
Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM53 PE
  • WM53_PE
    Human peripheral blood lymphocytes, monocytes and granulocytes stained with WM53 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
303403 25 tests 86€
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303404 100 tests 190€
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myeloid leukaemia cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Application References
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
  3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
  4. Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations
  1. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  2. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  3. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  4. Zhao Y, et al. 2020. Front Immunol. 2.572222222. PubMed
  5. Eriksson E, et al. 2019. J Immunol. 202:787. PubMed
  6. Sade-Feldman M, et al. 2016. Clin Cancer Res. 22(23):5661-5672. PubMed
  7. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed
  8. Schmiderer L, et al. 2020. Proc Natl Acad Sci U S A. 117:21267. PubMed
  9. Subramaniam A, et al. 2020. Blood. 136:2151. PubMed
  10. Danzer H, et al. 2020. Elife. 9:00. PubMed
  11. Salik B, et al. 2020. Cancer Cell. 38(2):263-278.e6. PubMed
  12. Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
  13. Tcheng M, et al. 2021. Blood. 137:3518. PubMed
  14. Han AR, et al. 2021. Int J Stem Cells. 14:203. PubMed
  15. Galán-Díez M, et al. 2022. Cancer Discov. . PubMed
  16. Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
  17. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  18. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  19. Lin G, et al. 2021. Mol Med Rep. 23:. PubMed
  20. Bhattacherjee A, et al. 2021. Mol Neurodegener. 16:19. PubMed
  21. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  22. Kenney DJ, et al. 2022. Cell Rep. 39:110714. PubMed
  23. Wu X, et al. 2023. iScience. 26:106559. PubMed
RRID
AB_314347 (BioLegend Cat. No. 303403)
AB_314348 (BioLegend Cat. No. 303404)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells

Function
Adhesion and lectin activity
Ligand/Receptor
Sugar chains containing sialic acid
Cell Type
B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 09-09-2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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