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PE anti-human CD335 (NKp46) Antibody

Pricing & Availability
Clone
9E2 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E2_PE_032808
Human peripheral blood lymphocytes stained with CD56 (HCD56) APC and 9E2 PE
  • 9E2_PE_032808
    Human peripheral blood lymphocytes stained with CD56 (HCD56) APC and 9E2 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
331907 25 tests 108€
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331908 100 tests 234€
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Description

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NKp46-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Application References
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.
Product Citations
  1. Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed
  2. Tognarelli S, et al. 2018. Front Immunol. 9:2743. PubMed
  3. Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed
  4. Mayassi T, et al. 2019. Cell. 176:967. PubMed
  5. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  6. Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
  7. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  8. Groen B, et al. 2015. Sci Rep. 5: 13618. PubMed
  9. Spallanzani R, et al. 2015. J Immunol. 195: 2141-2148. PubMed
  10. Zanetti S, et al. 2016. J Biol Chem. 291: 16541 - 16552. PubMed
  11. Sadallah S, et al. 2016. J Immunol. 197: 1663 - 1671. PubMed
  12. Rajashekar JK, et al. 2021. Cell Host Microbe. 29(6):904-916.e6. PubMed
  13. Lee Y, et al. 2020. J Innate Immun. 1:. PubMed
  14. Rademacher MJ, et al. 2021. Sci Rep. 11:8321. PubMed
  15. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  16. Ponath V, et al. 2021. Int J Mol Sci. 22:. PubMed
  17. Merah-Mourah F, et al. 2021. Curr Protoc. 1:e229. PubMed
  18. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
RRID
AB_1027679 (BioLegend Cat. No. 331907)
AB_1027666 (BioLegend Cat. No. 331908)

Antigen Details

Structure
Type I membrane glycoprotein (46 kD)
Distribution

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
9437 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04-15-2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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