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PE anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
F38-2E2 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_F38-2E2_PE_020810
Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom).
  • A_F38-2E2_PE_020810
    Th1-polarized human peripheral blood mononuclear cells were stained with CD4 FITC and anti-human CD366 (Tim-3, clone F38-2E2) PE (top) or mouse IgG1, κ PE (bottom).
  • B_F38-2E2_PE_020810
See PE spectral data
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345005 25 tests 123€
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345006 100 tests 288€
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Tim-3 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

Application References
  1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
  2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
  3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations
  1. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  2. Finney OC, et al. 2019. J Clin Invest. 130:. PubMed
  3. Yang H, et al. 2019. Mol Metab. 23:24. PubMed
  4. Gorris MAJ, et al. 2018. J Immunol. 200:347. PubMed
  5. Andersch L, et al. 2019. BMC Cancer. 19:895. PubMed
  6. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  7. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  8. Zhang Y, et al. 2012. J Leukoc Biol. 91:189. PubMed
  9. Wei F, et al. 2013. Proc Natl Acad Sci U S A. 110:2480. PubMed
  10. Fang L, et al. 2013. Neuro Oncology. 15:1479. PubMed
  11. Poonia B, Pauza C 2014. PLoS One. 9:88884. PubMed
  12. Fromentin R, et al. 2016. PLoS Pathog. 12: 1005761. PubMed
  13. Jin C, et al. 2016. EMBO Mol Med. 8: 702 - 711. PubMed
  14. Ho T, et al. 2016. Blood. 128: 1671 - 1678. PubMed
  15. Wang S, et al. 2021. Exp Ther Med. 21:37. PubMed
  16. Barresi V, et al. 2020. J Clin Med. 9:00. PubMed
  17. Bhattacharya P, et al. 2020. Elife. 9:00. PubMed
  18. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  19. Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
  20. Roberts A, et al. 2021. Sci Rep. 11:4030. PubMed
  21. Ho JY, et al. 2021. Mol Ther Methods Clin Dev. 21:237. PubMed
  22. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  23. Cai D, et al. 2021. Front Mol Biosci. 8:686803. PubMed
  24. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  25. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  26. Sun L, et al. 2020. J Diabetes Res. 2020:2583257. PubMed
  27. Jung IY, et al. 2022. Sci Transl Med. 14:eabn7336. PubMed
  28. Zhang T, et al. 2022. Am J Reprod Immunol. 87:e13524. PubMed
  29. Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed
  30. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  31. Ceppi F, et al. 2022. Cancer Immunol Res. 10:856. PubMed
  32. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  33. Gkika E, et al. 2023. NPJ Precis Oncol. 7:24. PubMed
  34. Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed
RRID
AB_1877236 (BioLegend Cat. No. 345005)
AB_2116576 (BioLegend Cat. No. 345006)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9
Cell Type
Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.

Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 5    Revision Date: 07-03-2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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