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PE anti-human IL-5 Antibody

Pricing & Availability
Clone
JES1-39D10 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
Isotype
Rat IgG2a, κ
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Product Citations
publications
JES1-39D10_PE_030408
Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with JES1-39D10 PE
  • JES1-39D10_PE_030408
    Enriched human CD4+ T cells were stimulated with PMA+ionomycin, then surface stained with CD4 PE/Cy5 and intracellular stained with JES1-39D10 PE
See PE spectral data
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500903 25 tests 84€
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500904 100 tests 196€
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant human IL-5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1-3 or ELISPOT4-6 Capture: The purified JES1-39D10 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the biotinylated JES1-5A10 (Cat. No. 501002) antibody as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500907).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 & 430404) are specially developed and recommended.

Application References
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Butterfield, J., et al. 1992. Blood 79:688.
  3. Abrams, J., et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker, New York. p.133.
  4. Klinman, D., et al. 1994. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.19.6.
  5. Mahanty, S., et al. 1992. J. Immunol. 148:3567.
  6. Limaye, A., et al. 1991. J. Clin. Inv. 88:1418.
  7. Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
  8. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  9. Andersson, J., et al. 1994. Immunology 83:16.
Product Citations
  1. Golebski K, et al. 2019. Nat Commun. 10:2162. PubMed
  2. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  3. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  4. Numazaki M, et al. 2021. J Pharmacol Exp Ther. :JPET-AR-2021-000686. PubMed
  5. Dai G, et al. 2021. JCI Insight. 6:. PubMed
RRID
AB_315138 (BioLegend Cat. No. 500903)
AB_315139 (BioLegend Cat. No. 500904)

Antigen Details

Structure
Cytokine; dimer; 45 kD (Mammalian)
Bioactivity
Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
Eosinophils
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Takatsu, K., et al. 1988. Immunol. Rev. 102:107.
3. Takatsu, K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu, K. 1991. Microbiol. Immun. 35:593.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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