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PE anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8.8_PE_CD326(EpCAM)_Antibody_1_FC_100416
TE-71 (mouse thymic epithelial stromal cell line) cells were stained with CD326 (clone G8.8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • G8.8_PE_CD326(EpCAM)_Antibody_1_FC_100416
    TE-71 (mouse thymic epithelial stromal cell line) cells were stained with CD326 (clone G8.8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
See PE spectral data
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118205 50 µg 82€
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118206 200 µg 264€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chiang HC, et al. 2018. Sci Rep. 8:2731. PubMed
  2. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  3. He B, et al. 2018. Nat Commun. 9:5240. PubMed
  4. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  5. Yin N et al. 2019. Cancer Cell. 36(2):156-167 . PubMed
  6. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  7. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  8. Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
  9. Fujino N, et al. 2019. J Exp Med. 216:2184. PubMed
  10. Filbey KJ, et al. 2019. Front Immunol. 10:2375. PubMed
  11. Edelson B, et al. 2011. PLoS One. 6:e25660. PubMed
  12. Kim S, et al. 2011. PLoS One. 6:e19310. PubMed
  13. Lee J, et al. 2013. Am J Respir Cell Mol Biol. 48:288. PubMed
  14. Yu D, et al. 2013. Mol Cell Biol. 33:1845. PubMed
  15. Pastor W, et al. 2014. Nat Commun. 12:5795. PubMed
  16. Shigeyasu K, et al. 2015. Gut. 64:627. PubMed
  17. Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
  18. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  19. Nair S, et al. 2016. Nat Commun. 7:10913. PubMed
  20. Tan C, et al. 2020. Front Cell Dev Biol. 8:579157. PubMed
  21. Yang Y, et al. 2021. Cell Reports. 34(10):108822. PubMed
  22. Valds-Mora F, et al. 2021. Cell Reports. 35(2):108945. PubMed
  23. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  24. Ichinose M, et al. 2021. Sci Rep. 7200:11. PubMed
  25. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  26. Parker KA, et al. 2021. Mol Cancer Res. 19:2068. PubMed
  27. Airik M, et al. 2020. Hum Mol Genet. 29:3064. PubMed
  28. Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
  29. Skibba ME, et al. 2021. Respir Res. 22:315. PubMed
  30. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  31. Li Q, et al. 2022. J Biol Chem. :101753. PubMed
  32. Hao F, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  33. Wang M, et al. 2022. Theranostics. 12:4513. PubMed
  34. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  35. Kudira R, et al. 2021. Bio Protoc. 11:e4192. PubMed
  36. Barr J, et al. 2022. Elife. 11:. PubMed
  37. Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed
  38. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  39. Jiang W, et al. 2022. Redox Biol. 56:102432. PubMed
  40. Naranjo S, et al. 2022. Genes Dev. 36:936. PubMed
  41. Yang H, et al. 2023. Cell Death Differ. 30:560. PubMed
  42. Pennitz P 2022. STAR Protocols. 4(1):101957. PubMed
RRID
AB_1134176 (BioLegend Cat. No. 118205)
AB_1134172 (BioLegend Cat. No. 118206)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 10-04-2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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