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PE/Cyanine7 anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_581_PECy7_100609
Human peripheral blood stained with 581 PE/Cyanine7 or mouse IgG1,k isotype control PE/Cyanine7. Cytograms were gated to display total live CD14 negative mononuclear cells.
  • A_581_PECy7_100609
    Human peripheral blood stained with 581 PE/Cyanine7 or mouse IgG1,k isotype control PE/Cyanine7. Cytograms were gated to display total live CD14 negative mononuclear cells.
  • B_581_PECy7_100609
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
343515 25 tests 113€
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343516 100 tests 264€
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Sun Z, et al. 2018. Oncotarget. 9:10945. PubMed
  2. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  3. Perna F, et al. 2017. Cancer Cell. 32:506. PubMed
  4. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  5. Gillissen MA, et al. 2018. Blood. 131:131. PubMed
  6. Koide M, et al. 2018. Tohoku J Exp Med. 244:15:00. PubMed
  7. Garciaz S, et al. 2019. Clin Epigenetics. 0.55625. PubMed
  8. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  9. Nafria M, et al. 2020. Cell Rep. 31:107691. PubMed
  10. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  11. Kraus H, et al. 2014. J Immunol. 192:1044. PubMed
  12. Ochi K, et al. 2014. Stem Cells Trans Med. 3:792. PubMed
  13. Yoshioka S 2014. Sci Rep. 4:5066. PubMed
  14. Hsieh W, et al. 2015. Gut. 64:312. PubMed
  15. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
  16. Ng E, et al. 2016. Nat Biotechnol. 34:1168-1179. PubMed
  17. Nafria M, et al. 2020. STAR Protoc. 1:100130. PubMed
  18. Stavish D, et al. 2020. Nat Commun. 3.922916667. PubMed
  19. Dyring-Andersen B, et al. 2020. Nat Commun. 4.338194444. PubMed
  20. Chou DB, et al. 2020. Nat Biomed Eng. 0.440277778. PubMed
  21. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  22. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  23. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  24. Richardson SE, et al. 2021. STAR Protocols. 2(2):100420. PubMed
  25. Herland A, et al. 2020. Nat Biomed Eng. 421:4. PubMed
  26. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  27. Amoah A, et al. 2021. Haematologica. Online ahead of print. PubMed
  28. Laustsen A, et al. 2021. Elife. 10:. PubMed
  29. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  30. Li X, et al. 2021. Hemodial Int. 25:489. PubMed
  31. Mishra P, et al. 2021. Indian J Hematol Blood Transfus. 37:391. PubMed
  32. Vanuytsel K, et al. 2022. Cytometry A. 101:903. PubMed
  33. Connor T, et al. 2022. Methods Mol Biol. 2475:47. PubMed
  34. Aru B, et al. 2023. Balkan Med J. 40:117. PubMed
  35. Calvanese V, et al. 2022. Nature. 604:534. PubMed
RRID
AB_1877252 (BioLegend Cat. No. 343515)
AB_1877251 (BioLegend Cat. No. 343516)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org
Go To Top Version: 2    Revision Date: 03-18-2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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