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PerCP/Cyanine5.5 anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
GL7_PerCPCyanine55_GL7_Antibody_1_031324
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7) PerCP/Cyanine5.5 (left) or rat IgM, κ PerCP/Cyanine5.5 isotype control (right).
  • GL7_PerCPCyanine55_GL7_Antibody_1_031324
    BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7) PerCP/Cyanine5.5 (left) or rat IgM, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
144609 25 µg 141€
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144610 100 µg 306€
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  2. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  3. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  4. Hong JP, et al. 2020. Cell Reports Medicine. 1(3):100035. PubMed
  5. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
  6. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  7. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  8. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  9. Wong R, et al. 2020. Immunity. 53(5):1078-1094.e7. PubMed
  10. Seydoux E, et al. 2021. Cell Reports. 35(5):109084. PubMed
  11. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  12. Brooks JF, et al. 2021. Cell Reports. 36(9):109645. PubMed
  13. Gary E, et al. 2020. Vaccine. 3821:38. PubMed
  14. Verheijen M, et al. 2020. Cell Rep. 108376:33. PubMed
  15. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  16. Rivas MA, et al. 2021. Nat Immunol. 22:240. PubMed
  17. Mathur S, et al. 2021. JCI Insight. 6:. PubMed
  18. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  19. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  20. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  21. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  22. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  23. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  24. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  25. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  26. Trindade BC, et al. 2021. Immunity. 54:2273. PubMed
  27. Suzuki Y, et al. 2022. Mol Ther Nucleic Acids. 30:226. PubMed
  28. Chaurio RA, et al. 2022. Immunity. 55:115. PubMed
  29. Lee JH, et al. 2022. Cell Rep. 38:110485. PubMed
  30. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
  31. Leung W, et al. 2022. Cancer Discov. 12:1782. PubMed
  32. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  33. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  34. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  35. Grenov A, et al. 2022. Cell Rep. 39:110778. PubMed
  36. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  37. Prior JT, et al. 2023. NPJ Vaccines. 8:80. PubMed
RRID
AB_2562978 (BioLegend Cat. No. 144609)
AB_2562979 (BioLegend Cat. No. 144610)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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