- Clone
- 2F1-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Actin, cytoplasmic 1
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
643801 | 25 µg | 81€ | ||||
643802 | 100 µg | 184€ |
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
Product DetailsProduct Details
- Reactivity
- Human,Mouse,Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol.
- Application References
-
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
- Yanagiya T,et al.2007.Obesity.15:572.
- KishidaT,et al. 2007.J. Immunol. 179:8554.
- Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
- Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
- Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
- Ikeda D, et al. 2008. Endocrinology. 149:6037.
- Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
- Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
- Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
- Product Citations
- RRID
-
AB_2223201 (BioLegend Cat. No. 643801)
AB_2223199 (BioLegend Cat. No. 643802)
Antigen Details
- Structure
- Actin, cytoplasmic 1
- Distribution
-
Ubiquitously expressed in the cytoplasm of all eukaryotic cells
- Function
- Actins are highly conserved proteins that are involved in cell motility
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microfilaments
- Antigen References
-
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687. - Gene ID
- 11461 View all products for this Gene ID
- UniProt
- View information about beta-actin on UniProt.org
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