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Purified anti-human CD11b Antibody

Pricing & Availability
Clone
ICRF44 (See other available formats)
Regulatory Status
RUO
Workshop
IV M047
Other Names
Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ICRF44_Purified_061407
Human peripheral blood granulocytes stained with purified ICRF44, followed by anti-mouse IgGs FITC
  • ICRF44_Purified_061407
    Human peripheral blood granulocytes stained with purified ICRF44, followed by anti-mouse IgGs FITC
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301302 100 µg 40€
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Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and factor X.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
ICC, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The ICRF44 antibody inhibits heterotypic adhesion of granulocytes in response to fMLP. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, immunofluorescence microscopy5, stimulation of monocytes3, blocking of heterotypic PMN aggregation8, and blocking of granulocyte activation12. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301361 & 301362).

Application References
  1. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  2. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  3. Rezzonico R, et al. 2001. Blood 97:2932. (Stim)
  4. Marsik C, et al. 2003. Shock 20:493. (FC)
  5. David A, et al. 2003. J. Leukoc. Biol. 74:551. (IF)
  6. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  7. Thurlow LR, et al. 2010. Infect. Immun. 128:1128. (FC) PubMed
  8. Jadhav S, et al. 2001. J. Immunol. 167:5986. (Block)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21. (FC)
  11. Wen T, et al. 2014. J Immunol. 192:5481. (FC) PubMed
  12. Sprong T, et al. 2003. Blood 102:3702. (Block)
  13. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
  14. Larsson K, et al. 2015. PNAS. PubMed
Product Citations
  1. Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
  2. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  3. Tseng HY, et al. 2018. J Cell Sci. 131. PubMed
  4. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
  5. Lu Y, et al. 2017. Cell Mol Immunol. 1.054166667. PubMed
  6. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  7. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  8. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  9. Zhou H, et al. 2017. Leukemia. 31:2065. PubMed
  10. Patel AA, et al. 2017. J Exp Med. 214:1913. PubMed
  11. Dixit N, et al. 2011. J Immunol. 187:472. PubMed
  12. Söderholm N, et al. 2011. PLoS One. 6:e24353. PubMed
  13. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
  14. Larsson K, et al. 2015. Proc Natl Acad Sci U S A. . PubMed
  15. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  16. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  17. De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
  18. Lukácsi S, et al. 2020. PLoS One. 15:e0232432. PubMed
  19. Chen X, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  20. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  21. Noura M, et al. 2020. Sci Rep. 10:20245. PubMed
  22. Nagy-Baló Z, et al. 2021. PLoS One. 16:e0254853. PubMed
  23. Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed
  24. Percivalle E, et al. 2021. Viruses. 13:. PubMed
  25. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  26. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  27. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  28. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  29. Kirolos SA, et al. 2022. J Leukoc Biol. 112:1399. PubMed
RRID
AB_314154 (BioLegend Cat. No. 301302)

Antigen Details

Structure
Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subset of T cells, subset of B cells

Function
Adhesion, phagocytosis, chemotaxis, neutrophil activation
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stewart M, et al. 1995. Curr Opin Cell Biol. 7:690.

Gene ID
3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

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