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Purified anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
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Product Citations
publications
6H6
Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
  • 6H6
    Human peripheral blood lymphocytes stained with 6H6 PE and CD45RO PE/Cy5
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306002 100 µg 81€
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Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
IHC-F, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Application References
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  2. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  3. Olin A, et al. 2018. Cell. 174:1277. PubMed
  4. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  5. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  6. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  7. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  8. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  9. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  10. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  11. Parackova Z, et al. 2020. Sci Rep. 0.759027778. PubMed
  12. Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
  13. Martin-Gayo E, et al. 2010. Blood. 115:5366. PubMed
  14. Chen S, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  15. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  16. Liu SQ, et al. 2021. Cancer Immunol Res. 9:34. PubMed
  17. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  18. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  19. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  20. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  21. Henrick BM, et al. 2021. Cell. . PubMed
  22. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  23. Kremenovic M, et al. 2020. Cancers (Basel). 12:. PubMed
  24. Saygin C, et al. 2021. Leukemia. 35:3406. PubMed
  25. NULL, et al. 2022. Cell. 185:916. PubMed
  26. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  27. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  28. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  29. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  30. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  31. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  32. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  33. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  34. Laggner M, et al. 2022. EBioMedicine. 81:104093. PubMed
  35. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
RRID
AB_314576 (BioLegend Cat. No. 306002)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 08-30-2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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