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Purified anti-human CD8a Antibody

Pricing & Availability
Clone
RPA-T8 (See other available formats)
Regulatory Status
RUO
Workshop
IV T171
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T8_Purified_060807
Human peripheral blood lymphocytes stained with purified RPA-T8, followed by anti-mouse IgGs FITC
  • RPA-T8_Purified_060807
    Human peripheral blood lymphocytes stained with purified RPA-T8, followed by anti-mouse IgGs FITC
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301002 100 µg 40€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Costim, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Application References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
  5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
  6. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  2. Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed
  3. Alroqi F, et al. 2017. J Clin Immunol. . 10.1007/s10875-017-0451-1. PubMed
  4. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  5. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  6. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  7. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  8. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  9. Nicolet BP, et al. 2017. J Immunol. 198:962. PubMed
  10. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  11. Martins MA, et al. 2017. AIDS Res Hum Retroviruses. 33:843. PubMed
  12. Silva–Cardoso SC, et al. 2017. J Immunol. 199:253. PubMed
  13. Eriksson E, et al. 2017. Clin Cancer Res. 5.018055556. PubMed
  14. Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
  15. Simon S, et al. 2018. Front Immunol. 1.7375. PubMed
  16. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  17. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  18. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  19. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  20. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
  21. Jouand N, et al. 2018. PLoS Pathog. 14:e1007041. PubMed
  22. Kong R, et al. 2019. Cell. 178:567. PubMed
  23. Tripathi T, et al. 2019. Immunohorizons. 0.201388889. PubMed
  24. Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
  25. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  26. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  27. Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
  28. Schwabenland M, et al. 2021. Immunity. . PubMed
  29. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  30. Martin E, et al. 2020. JCI Insight. :5. PubMed
  31. Yang Q, et al. 2020. J Immunol. 2331:204. PubMed
  32. Levin SD, et al. 2020. Front Immunol. 10:3086. PubMed
  33. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  34. Zheng Y, et al. 2022. iScience. 25:103785. PubMed
  35. Anna F, et al. 2021. J Immunother Cancer. 9:. PubMed
  36. Gao H, et al. 2021. J Virol. :. PubMed
  37. Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed
  38. O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
  39. Dubrovsky L, et al. 2020. MBio. 11:. PubMed
  40. Liu X, et al. 2022. STAR Protoc. 3:101310. PubMed
  41. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  42. Lu X, et al. 2021. J Exp Med. 218: . PubMed
  43. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  44. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  45. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  46. Feyaerts D, et al. 2022. Cell Rep Med. 3:100680. PubMed
  47. Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed
RRID
AB_314120 (BioLegend Cat. No. 301002)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 4    Revision Date: 08-24-2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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