- Clone
- RB6-8C5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Gr-1
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RB6-8C5_ALX647_020708 RB6-8C5_ALX647_020708](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RB6-8C5_ALX647_020708.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) Alexa Fluor® 647 (filled histogram) or rat IgG2b, κ Alexa Fluor® 647 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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108420 | 25 µg | 81€ | ||||
108418 | 100 µg | 184€ |
Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Raised against granulocytes of mouse origin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
SB - Community verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
-
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.
The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.
Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436). - Additional Product Notes
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This product has been verified for IHC-F (Immunohistochemistry - frozen tissue sections) on the NanoString GeoMx® Digital Spatial Profiler. The GeoMx® enables researchers to perform spatial analysis of protein and RNA targets in FFPE and fresh frozen human and mouse samples. For more information about our spatial biology products and the GeoMx® platform, please visit our spatial biology page.
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Application References
(PubMed link indicates BioLegend citation) -
- Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
- Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
- Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
- Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
- Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
- Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
- Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
- Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
- Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
- Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
- Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
- Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
- Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
- Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
- Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
- Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
- Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
- Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
- Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
- Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
- Collins C, et al. 2014. PNAS. 111:9899. PubMed
- Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
- Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
- Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
- Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
- Distel E, et al. 2014. Circ Res. 115:759. PubMed
- Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
- Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
- Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
- Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
- Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
- Product Citations
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- RRID
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AB_493481 (BioLegend Cat. No. 108420)
AB_389331 (BioLegend Cat. No. 108418)
Antigen Details
- Structure
- 21-25 kD
- Distribution
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Granulocytes, monocytes
- Cell Type
- Granulocytes, Monocytes, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Antigen References
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1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125. - Gene ID
- 17067 View all products for this Gene ID 546644 View all products for this Gene ID
- UniProt
- View information about Ly-6G Ly-6C on UniProt.org
Related FAQs
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
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It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
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Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
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Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
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Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
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In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
Other Formats
View All Ly-6G/Ly-6C Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with Ly-6G/Ly-6C APC (clon... -
Biotin anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with biotinylated Ly-6G/Ly... -
FITC anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with Ly-6G/Ly-6C (clone RB... -
PE anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
PE/Cyanine5 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with Ly-6G/Ly-6C (clone RB... -
Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with purified L... -
PE/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Alexa Fluor® 488 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population)... -
Alexa Fluor® 647 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population)... -
Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population)... -
Brilliant Violet 711™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
APC/Cyanine7 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow stained with CD11b FITC and Ly-6G/... -
Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population)... -
PerCP/Cyanine5.5 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow were stained with CD11b FITC and L... -
PerCP anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow (gated on myeloid cell population)... -
Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Brilliant Violet 570™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly6C... -
Ultra-LEAF™ Purified anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with LEAF™ puri... -
Brilliant Violet 510™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Brilliant Violet 605™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Brilliant Violet 650™ anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Alexa Fluor® 594 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse frozen lymph node section was fixed with 4% pa... C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Purified anti-mouse Ly-6G/Ly-6C (Gr-1) (Maxpar® Ready)
Mouse splenocytes stained with 141Pr anti-Ly-6G/C (RB6-8C5) ... -
PE/Dazzle™ 594 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
APC/Fire™ 750 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with CD11b PE a... -
TotalSeq™-A0116 anti-mouse Ly-6G/Ly-6C (Gr-1)
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TotalSeq™-C0116 anti-mouse Ly-6G/Ly-6C (Gr-1)
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TotalSeq™-B0116 anti-mouse Ly-6G/Ly-6C (Gr-1)
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Spark Blue™ 550 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow was stained with CD11b APC and Ly-... -
APC/Fire™ 810 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with Ly-6G/Ly-6... -
Spark Violet™ 423 anti-mouse Ly-6G/Ly-6C (GR-1) Antibody
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark UV™ 387 anti-mouse Ly-6G/Ly-6C (GR-1)
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
Spark Violet™ 538 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow were stained with anti-mouse CD11b... -
Spark PLUS UV™ 395 anti-mouse Ly-6G/Ly-6C (Gr-1)
C57BL/6 mouse bone marrow cells were stained with anti-mouse...
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