Purified anti-human CD24 Antibody

Pricing & Availability
Clone
ML5 (See other available formats)
Regulatory Status
RUO
Workshop
V CD24.5
Other Names
Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
ML5
Human whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC
  • ML5
    Human whole blood lymphocytes stained with purified ML5 and detected with anti-mouse IgGs FITC
  • ML5_IF_072512
    MCF-7 breast cancer cells were stained with anti-CD24 (clone ML5) followed by DyLight™ 649 Goat anti-mouse Ig secondary antibody (red), plus DAPI staining for nuclei (blue). Images were taken under 20x bin4 (Filter set: EX647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by Er Liu and John Nolan, La Jolla Institute for Bioengineering.
Cat # Size Price Quantity Check Availability Save
311101 25 µg 48€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
311102 100 µg 92€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
  2. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  3. Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
  4. Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Product Citations
  1. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  2. Olin A, et al. 2018. Cell. 174:1277. PubMed
  3. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  4. Yang SH, et al. 2019. Artif Cells Nanomed Biotechnol. 47:3021. PubMed
  5. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  6. Afzali B, et al. 2017. Nat Immunol. 18:813. PubMed
  7. Gruber CN, et al. 2020. Immunity. 53(3):672-684. PubMed
  8. Clark A, Petty H 2016. Sci Rep. 6:27039. PubMed
  9. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  10. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  11. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  12. Henrick BM, et al. 2021. Cell. . PubMed
  13. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  14. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  15. Jennelle LT, et al. 2021. Clin Transl Gastroenterol. 12:e00353. PubMed
  16. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
  17. Sigal N, et al. 2022. Methods Mol Biol. 2543:113. PubMed
  18. Jang G, et al. 2022. NPJ Genom Med. 7:63. PubMed
  19. McIlwain DR, et al. 2021. Cell Host Microbe. 29:1828. PubMed
  20. McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed
  21. Sanmarco LM, et al. 2019. JCI Insight. :4. PubMed
RRID
AB_314850 (BioLegend Cat. No. 311101)
AB_314851 (BioLegend Cat. No. 311102)

Antigen Details

Structure
GPI-linked glycoprotein, 35-45 kD
Distribution

B cells, granulocytes, epithelial cells

Function
B cell proliferation and differentiation
Ligand/Receptor
CD62P (P-Selectin)
Cell Type
B cells, Epithelial cells, Granulocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.

Gene ID
100133941 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.09.2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account