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APC anti-mouse CD62L Antibody

Pricing & Availability
Clone
MEL-14 (See other available formats)
Regulatory Status
RUO
Other Names
L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEL-14_APC_090707
C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • MEL-14_APC_090707
    C57BL/6 mouse splenocytes were stained with CD62L (clone MEL-14) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Compare all formats See APC spectral data
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104411 25 µg £46
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104412 100 µg £133
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Description

CD62L is a 74-95 kD glycoprotein also known as L-selectin, LECAM-1, Ly-22, LAM-1, and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. CD62L is important in lymphocyte homing to high endothelial venules (HEV) in peripheral lymph nodes and leukocyte "rolling" on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon cellular activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naïve, effector, and memory T cells. CD62L has been reported to interact with CD34, GlyCAM-1, and MAdCAM-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/eb mouse B lymphoma 38C-13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1-3, complement-dependent cytotoxicity4, in vivo and in vitro blocking of adhesion1-3,5, and immunohistochemical staining of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections6. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 104457-104462).

Application References

(PubMed link indicates BioLegend citation)
  1. Gallatin WM, et al. 1983. Nature 304:30. (IP, Block)
  2. Siegelman MH, et al. 1990. Cell 61:611. (IP, Block)
  3. Lewinsohn DM, et al. 1987. J. Immunol. 138:4313. (IP, Block)
  4. Iwabuchi K, et al. 1991. Immunobiology 182:161. (CMCD)
  5. Pizcueta P, et al. 1994. Am. J. Pathol. 145:461.
  6. Reichert RA, et al. 1986. J. Immunol. 136:3535. (IHC, FC)
  7. Olver S, et al. 2006. Cancer Res. 66:571.
  8. Fukushima A, et al. 2006. Invest. Ophthalmol. Vis. Sci. 47:657. PubMed
  9. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  10. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  11. Lee JW, et al. 2006. Nature Immunol. 8:181.
  12. Shigeta A, et al. 2008. Blood 112:4915 (FC) PubMed
  13. de Vries VC, et al. 2009. Am. J. Transplant. 9:2270 PubMed
Product Citations
  1. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  2. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  3. Ferrao Blanco MN, et al. 2022. Br J Pharmacol. 179:2771. PubMed
  4. Chakraborty P, et al. 2022. Cancer Res. 82:1969. PubMed
  5. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  6. Hu X, et al. 2022. EBioMedicine. 86:104333. PubMed
  7. Dölz M, et al. 2022. iScience. 25:105372. PubMed
  8. Lombard-Vadnais F, et al. 2023. iScience. 26:105852. PubMed
  9. Cowan J, et al. 2013. J Exp Med. 210:675. PubMed
  10. Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed
  11. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  12. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  13. Du J, et al. 2022. Sci Immunol. 7:eabo5407. PubMed
  14. Ou W, et al. 2023. Nat Commun. 14:392. PubMed
  15. Battistello E, et al. 2023. Mol Cell. 83:1216. PubMed
  16. Huang T, et al. 2023. Front Immunol. 14:1124152. PubMed
  17. Chen F, et al. 2023. Acta Biomater. 164:387. PubMed
  18. Li T, et al. 2023. Nat Commun. 14:2498. PubMed
  19. Bhaskar A, et al. 2023. iScience. 26:106644. PubMed
  20. Scieszka DP, et al. 2023. Respir Res. 24:138. PubMed
  21. Milighetti M, et al. 2023. iScience. 26:106937. PubMed
  22. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  23. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  24. Gabriely G, et al. 2021. iScience. 24:103347. PubMed
  25. Di Martile M, et al. 2020. J Immunother Cancer. 8:. PubMed
  26. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  27. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  28. Rezende R, et al. 2015. Nat Commun. 6: 8726. PubMed
  29. Wang J, et al. 2012. Blood. 120:1489. PubMed
  30. Martens R, et al. 2020. Nat Commun. 11:1114. PubMed
  31. Thi VAD, et al. 2019. Mol Cells. 42:869. PubMed
  32. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  33. Wei H, et al. 2021. Malar J. 20:89. PubMed
  34. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  35. Jiang W, et al. 2022. Front Immunol. 13:878832. PubMed
  36. Shapiro MR, et al. 2020. Front Immunol. 1.972222222. PubMed
  37. Ren S, et al. 2019. Oncol Rep. 41:1509. PubMed
  38. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  39. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  40. Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
  41. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  42. Zhao Y, et al. 2021. J Biol Chem. 297:101166. PubMed
  43. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  44. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  45. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  46. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  47. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  48. Wiese A, et al. 2017. PLoS One. 10.1371/journal.pone.0184956. PubMed
  49. Roy S, et al. 2020. Sci Rep. 10:10992. PubMed
  50. Li Y, et al. 2020. Nat Commun. 11:2781. PubMed
  51. Parriott G, et al. 2020. Immunology. 160:280. PubMed
  52. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  53. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  54. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  55. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  56. Yu Y, et al. 2022. Nat Commun. 13:6357. PubMed
  57. Phiwpan K, et al. 2015. PLoS One. 10:128198. PubMed
  58. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  59. Deng B, et al. 2022. J Immunother Cancer. 10:. PubMed
  60. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  61. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  62. Lu Y, et al. 2021. Gastroenterology. 161:575. PubMed
  63. Kondratieva T, et al. 2020. PLoS One. 15:e0239668. PubMed
  64. Chin AL, et al. 2021. Nat Commun. 12:5138. PubMed
  65. Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
  66. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  67. Ruer-Laventie J, et al. 2020. Bio Protoc. e3531:10. PubMed
  68. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  69. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  70. Yang W, et al. 2020. Methods Mol Biol. 2111:91. PubMed
  71. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  72. Kulkarni R, et al. 2021. PLoS One. 16:e0257191. PubMed
  73. Glassman CR, et al. 2021. eLife. 10:00. PubMed
  74. Fu X, et al. 2020. Aging (Albany NY). 12:15656. PubMed
  75. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  76. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  77. Cowan J, et al. 2014. J Immunol. 193:1204. PubMed
  78. White C, et al. 2015. J Immunol. 194:697. PubMed
  79. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  80. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  81. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  82. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  83. Morimoto Y, et al. 2018. Immunity. 49:134. PubMed
  84. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  85. Ma K, et al. 2022. iScience. 25:104347. PubMed
  86. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  87. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  88. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  89. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  90. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  91. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  92. Do-Thi VA, et al. 2021. Cancers (Basel). 13:. PubMed
  93. Jenkinson W, et al. 2012. PLoS One. 7:e53416. PubMed
  94. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  95. Liu YG, et al. 2019. FASEB J. 33:5018. PubMed
  96. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  97. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  98. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  99. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  100. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  101. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  102. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  103. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  104. Hoechst B, et al. 2016. J Immunol. 195:1517-1523. PubMed
  105. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  106. Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed
  107. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  108. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  109. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  110. Rochford I, et al. 2021. Am J Physiol Lung Cell Mol Physiol. 321:L686. PubMed
  111. Liu X, et al. 2021. Adv Sci (Weinh). 8:e2100233. PubMed
  112. Kooijman S, et al. 2015. Am J Physiol Heart Circ Physiol. 309: H646-H654. PubMed
  113. Lin JD, et al. 2020. Cell Host & Microbe. 27(5):830-840. PubMed
  114. Chakraborty P, et al. 2020. Cell Reports. 28(7):1879-1893.e7.. PubMed
  115. Parks CA, et al. 2019. Proc Natl Acad Sci U S A. 116:3136. PubMed
  116. Angela M, et al. 2016. Nat Commun. 7:13683. PubMed
  117. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  118. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  119. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  120. Jiang W, et al. 2021. Cell Rep. 37:110112. PubMed
  121. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  122. Yu Z, et al. 2021. Biomed Res Int. 2021:9958745. PubMed
  123. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  124. Louise V Webb et al. 2019. Immunity. 50(2):348-361 . PubMed
  125. Chen S, et al. 2018. Nat Commun. 9:5298. PubMed
RRID
AB_313098 (BioLegend Cat. No. 104411)
AB_313099 (BioLegend Cat. No. 104412)

Antigen Details

Structure
Selectin, 95 kD (neutrophils) or 74 kD (lymphocytes)
Distribution

Subsets of B and T cells, monocytes, granulocytes, subset of NK cells

Function
Lymphocyte homing to HEV, rolling on activated endothelium
Ligand/Receptor
CD34, GlyCAM-1, MAdCAM-1
Cell Type
B cells, Granulocytes, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kishimoto TK, et al. 1990. P. Natl. Acad. Sci. USA 87:2244.
3. Tedder TF, et al. 1995. J. Exp. Med. 181:2259.

Gene ID
20343 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD62L
Specificity Alt (DOES NOT SHOW ON TDS):
CD62L
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD62L on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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