- Clone
- 1RS1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sigma 38, rpoS, appR, katF, nur, otsX, sigS, RNA Polymerase
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320 1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1RS1_PURE_Ecoli-RNA-SigmaS_Antibody_1_030320.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Whole cell extracts (15 µg protein) from E. coli DH5α cells grown to logarithmic (log) or stationary (stat) phase were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.0625 µg/mL (1:8000 dilution) of purified anti-E. coli RNA Sigma S antibody (clone 1RS1) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Ponceau S Red was used to confirm equal protein loading. Lane M: Molecular weight marker.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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663705 | 25 µg | £70 | ||||
663706 | 100 µg | £174 |
Sigma transcription factor controlling the regulation of genes is required for protection against external stresses. Possibly, it affects the expression of katE (catalase HPII), xthA (endonuclease III), the appABC operon, and other genes by modifying the affinity of RNA polymerase for the promoters.
Product DetailsProduct Details
- Verified Reactivity
- E. coli
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Previously item# W0009 from Neoclone.
Clone 1RS1 does not react with Shigella, Vibrio, Pseudomonas, Rhodobacter, Agrobacterium, Streptococcus, Arthobacter, or Borrelia. It recognizes epitope 33-256.
Additional reported applications (for the relevant formats) include: functional assay. -
Application References
(PubMed link indicates BioLegend citation) -
- Nguyen LH, et al. 1993. Biochemistry 32:11112.
- RRID
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AB_2832841 (BioLegend Cat. No. 663705)
AB_2832842 (BioLegend Cat. No. 663706)
Antigen Details
- Structure
- Sigma S (σS) is a 38 kD transcription factor of E. coli RNA polymerase.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Gene ID
- 947210 View all products for this Gene ID
- UniProt
- View information about RNA Sigma S on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All RNA Sigma S Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Anti-E. coli RNA Sigma S | 1RS1 | WB,ELISA,FA |
Purified anti-E. coli RNA Sigma S | 1RS1 | WB,ELISA,FA |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Anti-E. coli RNA Sigma S
Western blot analysis of 50 ng of recombinant E. coli... -
Purified anti-E. coli RNA Sigma S
Whole cell extracts (15 µg protein) from E. coli D...
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