Human IFN-gamma ELISA MAX Standard

Pricing & Availability

Regulatory Status: RUO
Other Names: IFN-g ELISA Kit
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Product Citations: publications

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Size

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430101

5 plates

212€

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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details

Technical Data Sheet (pdf)

Kit Contents

Kit Contents

Human IFN-γ ELISA MAX™ Capture Antibody (200X)
Human IFN-γ ELISA MAX™ Detection Antibody (200X)
Human IFN-γ Standard
Avidin-HRP (1000X)

Product Details

Reactivity

Human

Application

ELISA

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application References

Manaster I, et al. 2008. J. Immunol. PubMed
Peng J, et al. 2005. J. Immunother. 28:599. PubMed
Zhou J, et al. 2014. PLoS One. 9:94075. PubMed
Sautto GA, et al. 2015. Gut. PubMed

Product Citations

Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
Ivagnes A, et al. 2017. Oncoimmunology. 7:e1386826. PubMed
Mahoney KM, et al. 2019. Cancer Immunol Immunother. 68:421. PubMed
Du Q, et al. 2018. J Immunol. 201:533. PubMed
Chabanon RM, et al. 2019. J Clin Invest. 129:1211. PubMed
Liu X, et al. 2020. Ther Adv Med Oncol. 12:1758835920910347. PubMed
Saraiva DP, et al. 2020. Front Oncol. 1.488194444. PubMed
Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
Manaster I, et al. 2008. J Immunol. 181:1869. PubMed
Zhou J, et al. 2014. PLoS One. 9:94075. PubMed
Sautto G, et al. 2015. Gut. . PubMed
Wang J, et al. 2020. Cell. 183:1264. PubMed
Landgraf KE, et al. 2020. Commun Biol. 0.330555556. PubMed
Chen L, et al. 2021. Int J Mol Sci. 22:. PubMed
Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
Sohail A, et al. 2022. PLoS Pathog. 18:e1010219. PubMed
Yang L, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
Wu J, et al. 2022. Mol Pharmacol. 102:161. PubMed
Hsiao CC, et al. 2022. STAR Protoc. 3:101422. PubMed
Martin A, et al. 2022. Mol Cancer Ther. 21:1184. PubMed
Xiao Q, et al. 2022. Sci Immunol. 7:eabl3995. PubMed
Asano T, et al. 2022. J Clin Lipidol. 17:78. PubMed
Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
Kang CE, et al. 2022. Sci Rep. 12:19030. PubMed

Standard Range

7.8-500 pg/mL

Materials Not Included

Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
A microplate reader capable of measuring absorbance at 450 nm
Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
Deionized (DI) water
Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
Wash bottle or automated microplate washer
TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
Stop Solution (2 N H₂SO₄)
Log-Log graph paper or software for data analysis
Tubes to prepare standard dilutions
Timer
Absorbent paper