ELISA MAX™ Standard Set Human TNF-α

Kit Contents

Kit Contents

• Human TNF-α ELISA MAX™ Capture Antibody (200X)
• Human TNF-α ELISA MAX™ Detection Antibody (200X)
• Human TNF-α Standard
• Avidin-HRP (1000X)

Product Details

Reactivity

Human

Application

ELISA

 

As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.

Application References

1. Davis AM, et al. 2008. J. Immunol. 180:6923. PubMed
2. Kulkarni O, et al. 2009. J. Pharmacol Exp Ther. 328:371. PubMed
3. Wang X, et al. 2014. Int J Cardiol. 174:688. PubMed
4. Sautto GA, et al. 2015. Gut. PubMed

Product Citations

1. Bikker R, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.06.009. PubMed
2. Kong B, et al. 2017. J Neuroinflammation. 10.1186/s12974-017-0961-z. PubMed
3. Sadewasser A, et al. 2019. Mol Ther Nucleic Acids. 16:686. PubMed
4. Khongkhunthian S, et al. 2018. Drug Discov Ther. 11:323. PubMed
5. Mamriev D, et al. 2020. Cell Death Dis. 0.79375. PubMed
6. Kulkarni O, et al. 2009. J Pharmacol Exp Ther. 328:371. PubMed
7. Davis A, et al. 2008. J Immunol. 180:6923. PubMed
8. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
9. Wang X, et al. 2014. Int J Cardiol. 174:6888. PubMed
10. Sautto G, et al. 2015. Gut. . PubMed
11. Grech D, et al. 2016. J Acupunct Meridian Stud. 9:234-241. PubMed
12. Jø nsson K, et al. 2017. Nat Commun. 8:14391. PubMed
13. Bibby J, et al. 2020. Nat Commun. 2.827777778. PubMed
14. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
15. Hoang QTM, et al. 2021. Front Med (Lausanne). 8:676058. PubMed
16. Ban T, et al. 2021. Nat Commun. 12:4379. PubMed
17. Rana T, et al. 2021. PLoS One. 16:e0246393. PubMed
18. Zeng W, et al. 2021. Exp Ther Med. 22:1186. PubMed
19. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
20. Sohail A, et al. 2022. PLoS Pathog. 18:e1010219. PubMed
21. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
22. Veerasubramanian PK, et al. 2021. Bioengineering (Basel). 9:. PubMed
23. Nakatomi K, et al. 2021. Biomed Rep. 14:21. PubMed
24. Chen S, et al. 2021. Exp Ther Med. 22:1049. PubMed
25. Palacios Y, et al. 2021. Int J Mol Sci. 22:. PubMed

Standard Range

7.8-500 pg/mL

Materials Not Included

• Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
• A microplate reader capable of measuring absorbance at 450 nm
• Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
• Deionized (DI) water
• Coating Buffer: 8.4 g NaHCO₃, 3.56 g Na₂CO₃, add DI H₂O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
• Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
• PBS: 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
• Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
• Wash bottle or automated microplate washer
• TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
• Stop Solution (2 N H₂SO₄)
• Log-Log graph paper or software for data analysis
• Tubes to prepare standard dilutions
• Timer
• Absorbent paper

Antigen Details

Cell Sources

Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells

Cell Type

Tregs

Biology Area

Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Gene ID

7124 View all products for this Gene ID

UniProt

View information about TNF-alpha on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis