7-AAD Viability Staining Solution

Pricing & Availability
Other Names
Viability dye, dead cell exclusion dye, cell viability dye
Ave. Rating
3 reviews
Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Product Details
Cat # Size Price Quantity Avail. Save
420403 200 tests $25.00
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420404 500 tests $50.00
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Technical data sheet

Product Details

Formulation
PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation
CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration
50 µg/ml
Storage & Handling
Protect from light. Store between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.

Application References

(PubMed link indicates BioLegend citation)

1. Paterson AM, et al. 2011. J. Immunol. 187:1097. PubMed
2. Wingren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000. PubMed
3. Sandu SK, et al. 2012. PNAS 109:20047. PubMed
4. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
5. Choudhury KR, et al. 2012. PLoS One. 7:e51415. PubMed
6. Wu Y, et al. 2013. J. Immunol. 190:1859. PubMed
7. Harker KS, et al. 2013. J. Leukoc Biol. 93:789. PubMed
8. Zhang ZN, et al. 2013. Clin Chem. 59:1175. PubMed
9. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
10. Cohen HB, et al. 2013. Blood. 122:1935. PubMed
11. Hufbauer M, et al. 2013. J. Virol. 87:12158. PubMed
12. Turner JE, et al. 2103. J Exp Med. PubMed
13. Teng T, et al. 2013. Mol Cell Biol. 33:4660. PubMed
14. Hussain MS, et al. 2013. Hum Mol Genet. 22:5199. PubMed
15. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
16. Kong W, et al. 2014. Cell Immunol. 287:1. PubMed
17. Fell LH, et al. 2014. Nephrol Dial Transplant. PubMed
18. Kraaji MD, et al. 2014. Cytokine. 67:7. PubMed
19. Schneider C, et al. 2014. PNAS. 111:8185. PubMed
20. Ermann J, et al. 2014. PNAS. 111:2559. PubMed
21. Cao L, et al. 2014. J Virol. 88:8656. PubMed
22. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
23. Wedel J, et al. 2014. J Leukoc Biol. 96:453. PubMed
24. Jaworska J, et al. 2015. J Immunol. 194:325. PubMed

Publication Library

Antigen Details

Antigen References

1. Schmid I, et al. 1992. Cytometry. 13:204

Gene ID
NA
UniProt
View information about 7-AAD on UniProt.org

Related FAQs

Why washing is not recommended post 7AAD or PI addition for viability check?
These dyes bind in equilibrium with DNA. Therefore external dye concentration must be maintained during analysis and the dye should not be washed out.
Go To Top Version: 2    Revision Date: 05/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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