Annexin V Binding Buffer

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Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution.

Product Details
Cat # Size Price Quantity Avail. Save
422201 100 ml $30.00
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Technical data sheet

Product Details

Storage & Handling
This product is guaranteed for nine months. Store between 2°C and 8°C.

FC - Quality tested
IF - Reported in the literature

Recommended Usage

Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml and then combined with Annexin V reagents at the recommended volumes.

Application Notes

Annexin V Staining
1. Wash cells twice with cold BioLegend cell staining buffer (cat # 420201) and then resuspend cells in Annexin V Binding Buffer (cat # 422201) at a concentration of 1x106 cells/ml.
2. Transfer 100 µl of cell suspension in 5 ml test tube.
3. Add 5 µl of fluorochrome conjugated Annexin V.
4. Add 10 µl of PI solution (cat # 421301) or 7-AAD (cat # 420403/420404).
5. Gently vortex the cells and incubate for 15 min at RT (25 °C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer (cat # 422201) to each tube. Analyze by flow cytometry.

Application References

(PubMed link indicates BioLegend citation)

1. Vermes I, et al. 1995. J. Immunol. Methods 184:39.
2. Soon PS, et al. 2013. Endocr Relat Cancer. 20:1. PubMed
3. Turner JE, et al. 2013. J Exp Med. PubMed
4. Lindner JM, et al. 2013. Mol Cell Biol. 33:4628. PubMed
5. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
6. Beggs KM, et al. 2014. Toxicol Sci. 137:91. PubMed
7. Miyazawa M, et al. 2014. Mol Biol Cell. 25:2116. PubMed
8. Burbulla LF, et al. 2014. Cell Death Dis. 5:1180. PubMed
9. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
10. Sahin E, 2014. J. Immunol. 193:1717. PubMed
11. Xiong R, et al. 2014. Toxicol Appl Pharmacol. 280:285. PubMed
12. Thakran S, 2015. Invest Ophthalmol Vis Sci. 56:177. PubMed

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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