Accutase® Cell Detachment Solution

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Other Names
Accutase® Cell Dissociation Reagent
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Accutase_Cell_Detachment_Solution_1_092013
Human umbilical vein endothelial cells, HUVEC, were detached with Accutase® Cell Detachment Solution (Cat. No. 423201) and stained with CD144 (VE-Cadherin) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram). Data was analyzed with 7-amino-actinomycin D (7-AAD) (Cat. No. 420404) live/dead cell discrimination.
  • Accutase_Cell_Detachment_Solution_1_092013
    Human umbilical vein endothelial cells, HUVEC, were detached with Accutase® Cell Detachment Solution (Cat. No. 423201) and stained with CD144 (VE-Cadherin) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram). Data was analyzed with 7-amino-actinomycin D (7-AAD) (Cat. No. 420404) live/dead cell discrimination.
  • Accutase_Cell_Detachment_Solution_2_092013
Description

Accutase® is a cell detachment solution containing proteolytic and collagenolytic enzymes, which are neither mammalian nor bacterial derived. It is a replacement for Trypsin-EDTA solution and is useful for the detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, and polymers. Accutase® works exceptionally well for detaching cell lines for continued cell culturing, analysis of cell surface markers, virus growth assays, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxis, and tumor cell migration assays. Cell lines tested for Accutase™ application include fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and HNK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, MIC5, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251, D54, HT1080 fibrosarcoma cells, HT29 colon cancer cells, Sf9 insect cells, human embryonic stem cells, human mesenchymal stem cells, and human neural stem cells.

Product Details
Cat # Size Price Quantity Avail. Save
423201 100 ml $29.00
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Technical data sheet

Product Details

Formulation
Accutase® enzymes in Dulbecco's PBS containing 0.5 mM EDTA and phenol red.
Storage & Handling
This product is shipped with an ice pack. It may thaw during shipment. Upon receipt, store at -20°C. It can also be stored between 2°C and 8°C for up to 2 months.
Application

FC - Quality tested

Application Notes

Recommended Protocol:
1. Thaw Accutase® at 4°C or in a cool water bath (do not thaw at 37°C).
2. Aspirate the media and wash cells once with 1X Dulbecco's PBS (without calcium and magnesium).
3. Add an adequate volume of Accutase® Cell Detachment Solution to cover cells.
4. Allow cells to detach at room temperature or 37°C for 5 to 10 minutes, or until the cells are detached.
5. Strike the flask against palm of hand to detach cells.
6. Count cells and then resuspend in fresh medium to continue cell culture (in general, there is no neutralization step needed) or harvest the cells for other experimental needs.

Accutase is a registered trademark of Innovative Cell Technologies, Inc.

Antigen Details

Antigen References

1. Bajpai R, et al. 2008. Mol. Reprod. Dev. 75:818.
2. Yang D, et al. 2008. Stem Cells 26:55.
3. Brevig T, et al. 2006. Artif. Organs. 30:915.

Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 09/23/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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