- Clone
- M1/69 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Heat Stable Antigen (HSA), Ly-52, Nectadrin
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M1slash69_BV421_062311 M1slash69_BV421_062311](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M1slash69_BV421_062311.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Brilliant Violet 421™ (filled histogram) or with rat IgG2b Brilliant Violet 421™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
101825 | 125 µL | $171 | ||||
101826 | 50 µg | $237 |
CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101845 and 101846).
-
Application References
(PubMed link indicates BioLegend citation) -
- Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
- Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
- Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
- Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
- Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
- Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
- Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
- Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
- Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
- Product Citations
-
- RRID
-
AB_10901159 (BioLegend Cat. No. 101825)
AB_2563508 (BioLegend Cat. No. 101826)
Antigen Details
- Structure
- GPI-linked sialoglycoprotein, 35-45 kD
- Distribution
-
B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
- Function
- Regulates B cell proliferation and differentiation
- Ligand/Receptor
- P-selectin, CD24
- Cell Type
- B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437. - Gene ID
- 12484 View all products for this Gene ID
- UniProt
- View information about CD24 on UniProt.org
Related FAQs
- What is the F/P ratio range of our BV421™ format antibody reagents?
-
It is lot-specific. On average it ranges between 2-4.
Other Formats
View All CD24 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-mouse CD24
C57BL/6 splenocytes stained with biotinylated M1/69, followe... -
FITC anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 FITC -
PE anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 PE -
Purified anti-mouse CD24
C57BL/6 mouse splenocytes stained with purified M1/69, follo... -
Brilliant Violet 510™ anti-mouse CD24
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Bri... -
PE/Cyanine5 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 PE/Cyanine5 -
APC anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 APC -
Alexa Fluor® 488 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 Alexa Fluor®... -
Alexa Fluor® 647 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 Alexa Fluor®... -
Pacific Blue™ anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 Pacific Blue&tr... -
PE/Cyanine7 anti-mouse CD24
C57BL/6 mouse splenocytes stained with M1/69 PE/Cyanine7 -
Alexa Fluor® 594 anti-mouse CD24
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
PerCP/Cyanine5.5 anti-mouse CD24
C57 splenocytes stained with M1/69 PerCP/Cyanine5.5 -
Brilliant Violet 421™ anti-mouse CD24
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Bri... -
Brilliant Violet 605™ anti-mouse CD24
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Bri... -
Purified anti-mouse CD24 (Maxpar® Ready)
C57BL/6 mouse splenocytes stained with 176Yb-anti-CD45R/B220... -
PE/Dazzle™ 594 anti-mouse CD24
C57BL/6 splenocytes were stained with CD24 (clone M1/69) PE/... -
Alexa Fluor® 700 anti-mouse CD24
C57BL/6 splenocytes were stained with CD24 (clone M1/69) Ale... -
APC/Fire™ 750 anti-mouse CD24
C57BL/6 mouse splenocytes were stained with CD24 (clone M1/6... -
TotalSeq™-A0212 anti-mouse CD24
-
TotalSeq™-C0212 anti-mouse CD24
-
TotalSeq™-B0212 anti-mouse CD24
-
Ultra-LEAF™ Purified anti-mouse CD24
C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified ... -
APC/Cyanine7 anti-mouse CD24 Antibody
C57BL/6 splenocytes were stained with CD19 FITC and anti-mou... -
Brilliant Violet 711™ anti-mouse CD24
C57BL/6 splenocytes were stained with anti-mouse CD19 FITC a...
Follow Us