FITC anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
HI98 (See other available formats)
Workshop
IV M141
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgM, κ
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Product Citations
publications
HI98
Human peripheral blood granulocytes stained with HI98 FITC
  • HI98
    Human peripheral blood granulocytes stained with HI98 FITC
See FITC spectral data
Cat # Size Price Quantity Avail. Save
301903 25 tests $70
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301904 100 tests $160
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL), also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells and some malignant cells. CD15 has been implicated in adhesion, as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HI98 has been described to specifically react with 3-fucosyl-N-acetyllactosamine (3-FAL), also called X-hapten, SSEA-1, CD15, or Lewis X. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections5 and formalin-fixed paraffin-embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. van Baarsen LG, et al. 2014. Arthritis Res. Ther. 16:426. (IHC)
Product Citations
  1. Grenga I, et al. 2016. Clin Transl Immunology. 0.265972222. PubMed
  2. Aagaard K, et al. 2017. Sci Rep. 7:41389. PubMed
  3. Nechama M, et al. 2018. Nat Commun. 9:1603. PubMed
  4. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  5. Balogh P, et al. 2019. Haematologica. 105:00:00. PubMed
RRID
AB_314195 (BioLegend Cat. No. 301903)
AB_314196 (BioLegend Cat. No. 301904)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Monocytes, Neutrophils, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/19/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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