FITC Streptavidin

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Other Names
Streptavidin-Fluorescein Isothiocyanate, SAv-FITC
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SAV_FITC_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
  • SAV_FITC_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by Sav-FITC
See FITC spectral data
Description

Streptavidin binds to biotin with high affinity. Streptavidin-fluorescein isothiocyanate (FITC) is useful for detecting biotinylated antibodies. The excitation of FITC by 488 nm laser light induces a light emission maximum of 520 nm.

Product Details
Cat # Size Price Quantity Avail. Save
405201 100 µg $35.00
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405202 500 µg $100.00
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Technical data sheet

Product Details

Reactivity
Mouse, Human, Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-FITC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the FITC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Streptavidin-FITC is useful as a second step reagent for indirect immunofluorescent staining, in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-FITC is 60 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)

1. Schmutz S, et al. 2009. J. Immunol. 183:2217. PubMed
2. Cho HY, et al. 2009. Immunol Lett. 127:39. PubMed
3. de Wispelaere D, et al. 2012. J. Virol. 86:7072. PubMed
4. Fukuoka A, et al. 2013. Int Immunol. PubMed
5. Giltiay NV, et al. 2013. J Exp Med. 25:373. PubMed
6. Lee JH, et al. 2014. J Rheumatol. 41:2425. PubMed

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