PE Streptavidin

Pricing & Availability
Other Names
Streptavidin-Phycoerythrin, SAv-PE
Ave. Rating
1 reviews
SAV_PE_082808.jpg
C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
  • SAV_PE_082808.jpg
    C57BL/6 splenocytes stained with 145-2C11 biotin, followed by SAV-PE
See PE spectral data
Description

Streptavidin binds to biotin with high affinity. Streptavidin-Phycoerythrin (PE) is useful for detecting biotinylated antibodies. The excitation of PE by 488 nm laser light induces a light emission maximum of 575 nm.

Product Details
Cat # Size Price Quantity Avail. Save
405203 100 µg $45.00
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405204 500 µg $160.00
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Technical data sheet

Product Details

Reactivity
Mouse, Human, Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with PE under optimal conditions. The solution is free of unconjugated PE.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The streptavidin-PE solution should be stored undiluted between 2°C and 8°C, and protected from exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PE conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.015 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Streptavidin-Phycoerythrin (PE) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-PE is 292kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)

1. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245.
2. Nishimoto KP,et al. 2008. J. Immunol. 181:4010.PubMed
3. Niki T, et al. 2009. J. Biol. Chem. 284:32344. PubMed
4. Shibui A, et al. 2011. Exp Parasitol. 129:318. PubMed
5. Scatizzi JC, et al. 2012. J. Immunol. 188:3307. PubMed
6. Yamakawa N, et al. 2012. Int Immunol. 25:45. PubMed
7. Shibata T, et al. 2012. Int Immunol. 24:613. PubMed
8. Baccala R, et al. 2012. J. Immunol. 189:5976. PubMed
9. Grevers LC, et al. 2013. Ann Reheum Dis. 72:278. PubMed
10. Woo SJ, et al. 2013. J Leukoc Biol. 93:363. PubMed
11. Ashbaugh JJ, et al. 2013. J. Immunol. 190:4525. PubMed
12. Reading JL, 2013. J. immunol. 190:4542. Pubmed.
13. Kanno A, et al. 2013. Int Immunol. 25:413. PubMed
14. Gunaydin G, et al. 2014. Vaccine. 32:470. PubMed
15. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
16. Datta S, et al. 2014. J Leukoc Biol. 95:853. PubMed
17. He X, et al. 2014. J Immunol. 193:1314. PubMed
18. Wang W, et al. 2014. PNAS. 111:14466. PubMed
19. Murakami Y, et al. 2014. J Immunol. 193:5208. PubMed
20. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
21. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed
22. Hua X, et al. 2015. PloS One. 10:128039. PubMed

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