- Other Names
- Fixative, Paraformaldehyde
- Ave. Rating
- 2 reviews
Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.Product Details
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- Storage & Handling
- This buffer solution should be stored between 2°C and 8°C.
ICFC - Quality tested
- Recommended Usage
For cell fixation, use 0.5 ml fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.
- Application Notes
This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.
(PubMed link indicates BioLegend citation)
1. Kang YJ, et al. 2007. Nature Immunol. 8:601.Publication Library
2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
- Antigen References
1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
- Gene ID
- View information about Fixation Buffer on UniProt.org
- What is the difference between Paraformaldehyde and Alcohol mediated fixation and permeabilization?
- Alcohol treatment such as with ethanol or methanol both fixes as well as permeabilizes the cells (one step fix/perm process) and this treatment is a bit stronger than the conventional detergent based permeabilization and aldehyde based fixation (two step process) methods. It all depends on the type and location of your target. In order to access nuclear targets, a stronger permeabilization regimen may be required as compared to a target in cytoplasm. Alcohol is typically used to permeabilize cells in order to detect phosphorylated proteins and transcription factors because it can increase the reactivity of antibodies to certain nuclear antigens.