Loading...

Fixation Buffer

Pricing & Availability
Regulatory Status
RUO
Other Names
Fixative, Paraformaldehyde
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Check Availability Save
420801 100 mL $59
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Fixation Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Fixation Buffer is used to fix cells prior to permeabilization using Permeabilization Wash Buffer (Cat. No. 421002). BioLegend's Fixation Buffer has been formulated with prescreened paraformaldehyde with low background, thus producing the greatest signal to noise ratio.

Product Details
Technical data sheet

Product Details

Storage & Handling
This buffer solution should be stored between 2°C and 8°C.
Application

ICFC - Quality tested
ICC
Recommended Usage

For cell fixation, use 250 µL fixation buffer per tube and leave it in the dark for 20 minutes at room temperature. It is recommended that the reagent be titrated for optimal performance for each application. For the fixation procedure, please refer to the "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Caution: This buffer contains paraformaldehyde, which is toxigenic and mutagenic. Please handle with caution and wear gloves, lab coat and necessary protection to avoid direct body contacts.

Application Notes

This 1X PBS solution contains 4% paraformaldehyde, which is toxic and is a suspected carcinogen. Contact with eyes, skin and mucous membranes should be avoided.

Additional Product Notes

View more applications data using this product to stain Veri-Cells™ lyophilized control cells and to perform phospho-flow in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Kang YJ, et al. 2007. Nature Immunol. 8:601.
  2. Kenna TJ, et al. 2010. J. Immunol. 184:598. PubMed
  3. Sullivan BP, et al. 2010. Am J Pathol. 177:2837. PubMed
  4. del Rio ML, et al. 2011.Transplantation. 92:1085. PubMed
  5. del Rio ML, et al. 2012. J. Immunol. 188:4885. PubMed
  6. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  7. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  8. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
Product Citations
  1. Salybekov AA, et al. 2018. PLoS One. 13:e0203244. PubMed
  2. Haberthur K, et al. 2013. J Virol. 87:11751. PubMed
  3. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  4. Darzianiazizi M, et al. 2020. Int J Mol Sci. 21:00. PubMed
  5. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  6. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  7. Tocheva AS, et al. 2020. Curr Protoc Immunol. 130:e103. PubMed
  8. Shen Z, et al. 2020. Bioact Mater. 5:1113. PubMed
  9. Kim J, et al. 2021. Cytometry A. 99:807. PubMed
  10. Stoycheva D, et al. 2021. Eur J Immunol. 51:1423. PubMed
  11. Bruno MEC, et al. 2022. J Cell Physiol. 237:706. PubMed
  12. Mills EL, et al. 2022. Cell Metab. 34:140. PubMed
  13. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  14. Madi A, et al. 2022. Int J Cancer. 151:797. PubMed
  15. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  16. Sulaj A, et al. 2022. J Clin Endocrinol Metab. 107:2167. PubMed
  17. Brog RA, et al. 2022. Cancer Immunol Res. 10:962. PubMed
  18. Chen ML, et al. 2022. Mol Cancer Ther. 21:1337. PubMed
  19. Yin XY, et al. 2023. Neural Regen Res. 18:213. PubMed
  20. Gutierrez WR, et al. 2022. JCI Insight. 7: . PubMed
  21. Wang Y, et al. 2022. Front Endocrinol (Lausanne). 13:1036517. PubMed
  22. Koutník J, et al. 2022. Front Immunol. 13:1049033. PubMed
  23. Minott JA, et al. 2022. Front Immunol. 13:1038340. PubMed
  24. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  25. Noh J, et al. 2022. Cancers (Basel). 14: . PubMed
  26. Phares TW, et al. 2022. Front Pharmacol. 13:1029636. PubMed
  27. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  28. Chan L, et al. 2022. Cells. 12: . PubMed
  29. Qin S, et al. 2023. Acta Pharm Sin B. :. PubMed
  30. Gong HH, et al. 2023. Front Immunol. 13:1089064. PubMed
  31. Teplensky MH, et al. 2023. Nat Biomed Eng. :. PubMed
  32. Sun L, et al. 2023. Mol Med Rep. 27: . PubMed
  33. Chan L, et al. 2023. Int J Mol Sci. 24: . PubMed
  34. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  35. Xiao JF, et al. 2022. Mol Cancer Res. 20:637. PubMed
  36. Zander R, et al. 2022. Immunity. 55:475. PubMed
  37. Zhang H, et al. 2022. J Exp Med. 219:. PubMed
  38. Bronge M, et al. 2022. Sci Adv. 8:eabn1823. PubMed
  39. Zhuo Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  40. Tipanee J, et al. 2022. Mol Ther. 30:3155. PubMed
  41. Tang J, et al. 2022. Sci Immunol. 7:eadd4853. PubMed
  42. de Groot AE, et al. 2022. Neoplasia. 32:100830. PubMed
  43. Hajnik RL, et al. 2022. Sci Transl Med. 14:eabq1945. PubMed
  44. Frost JN, et al. 2022. Sci Adv. 8:eabq5384. PubMed
  45. Li L, et al. 2022. Cancer Immunol Res. 10:1475. PubMed
  46. Chernozem RV, et al. 2023. Adv Healthc Mater. 12:e2201726. PubMed
  47. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  48. Kodama N, et al. 2023. Front Nutr. 10:1081263. PubMed
  49. Humphries DC, et al. 2023. Front Immunol. 14:1100161. PubMed
  50. Wang H, et al. 2023. Nature. 615:339. PubMed
  51. Gayet RV, et al. 2023. Nat Commun. 14:1339. PubMed
  52. Varun K, et al. 2023. EBioMedicine. 90:104516. PubMed
  53. Wang YH, et al. 2023. Int J Mol Sci. 24:. PubMed
  54. Deng G, et al. 2023. Cancers (Basel). 15:. PubMed
  55. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  56. Xie J, et al. 2023. Mol Ther Oncolytics. 29:61. PubMed
  57. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  58. Ruan H, et al. 2023. Acta Pharm Sin B. 13:2202. PubMed
  59. Zhang TR, et al. 2023. Pharmaceuticals (Basel). 16:. PubMed
  60. Shou J, et al. 2023. Sci Adv. 9:eade9118. PubMed
  61. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  62. Li Q, et al. 2022. Cell Rep. 40:111308. PubMed
  63. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  64. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  65. Zhang L, et al. 2021. J Immunol. 207:590. PubMed
  66. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  67. Lentucci C, et al. 2017. J Biol Chem. 292:2754-2772. PubMed
  68. Ede J, et al. 2015. Toxicol Sci. 148: 108 - 120. PubMed
  69. Spitzer SO, et al. 2019. Neuron. 101:459. PubMed
  70. Li L,et al. 2017. Sci Rep.. 10.1038/s41598-017-14000-z. PubMed
  71. Kuo T, et al. 2017. Oncogene. 10.1038/onc.2017.156. PubMed
  72. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  73. Busskamp V, et al. 2014. Mol Syst Biol. 10:760. PubMed
  74. Jimenez-Duran G, et al. 2020. EBioMedicine. 61:103039. PubMed
  75. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  76. Lownik JC, et al. 2020. Biochem Biophys Res Commun. 522:442. PubMed
  77. Garton J, et al. 2021. Immunohorizons. 5:802. PubMed
  78. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  79. Stegelmeier AA, et al. 2022. Biomedicines. 10:. PubMed
  80. Thaker YR, et al. 2022. Front Oncol. 12:884196. PubMed
  81. de Groot AE, et al. 2021. Prostate. 81:629. PubMed
  82. Zhang S, et al. 2022. Cell Death Dis. 13:844. PubMed
  83. Bawazir M, et al. 2022. Front Immunol. 13:1033794. PubMed
  84. Tsai CY, et al. 2022. J Biomed Sci. 29:47. PubMed
  85. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  86. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  87. Brooks JF, et al. 2020. J Immunol. 205:1239. PubMed
  88. Jenull S, et al. 2021. Cell Reports. 36(3):109406. PubMed
  89. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  90. Wright A, et al. 2015. Immunobiology. 220: 859-864. PubMed
  91. Martinez-Usatorre A, et al. 2020. Front Immunol. 11:340. PubMed
  92. Martinez–Usatorre A, et al. 2019. Cancer Immunol Res. 0.995138889. PubMed
  93. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  94. Haryadi L, et al. 2019. Vet World. 12:1529. PubMed
  95. Guye P, et al. 2016. Nat Commun. 7:10243. PubMed
  96. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  97. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  98. Herda S, et al. 2021. Int J Cancer. 148:3097. PubMed
  99. Bayer AL, et al. 2022. Cancers (Basel). 14:. PubMed
  100. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  101. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  102. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  103. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  104. Wu Q, et al. 2022. Cell Mol Life Sci. 79:137. PubMed
  105. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  106. Shamsi F, et al. 2021. Nat Metab. 3:485. PubMed
  107. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  108. Klepsch V, et al. 2020. Cell Commun Signal. 18:08. PubMed
  109. Sullivan B, et al. 2010. Am J Pathol. 177:2837. PubMed
  110. Cheng K, et al. 2021. Nat Commun. 12:2041. PubMed
  111. Zhang B, et al. 2022. J Exp Med. 219:. PubMed
  112. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  113. Huang M, et al. 2022. Adv Sci (Weinh). 9:e2101267. PubMed
  114. Li FJ, et al. 2022. PLoS One. 17:e0277019. PubMed
  115. Takahashi K, et al. 2022. Cell Rep Methods. 2:100155. PubMed
  116. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  117. Rada M, et al. 2021. Commun Biol. 4:950. PubMed
  118. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  119. Leonard NA, et al. 2021. Cancers (Basel). 13:. PubMed
  120. Chen L, et al. 2021. Cell Death Differ. 28:1880. PubMed
  121. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  122. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  123. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  124. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  125. Elliott PR, et al. 2021. Cell Rep. 37:109777. PubMed
  126. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  127. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  128. Li J, et al. 2022. J Tissue Eng. 13:20417314221127908. PubMed
  129. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  130. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  131. Yang J, et al. 2022. Microbiome. 10:149. PubMed
  132. Alvandi Z, et al. 2022. J Am Heart Assoc. 11:e023695. PubMed
  133. van Duijn A, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  134. Jakic B, et al. 2021. Cell Death Dis. 12:187. PubMed
  135. Lamb C, et al. 2016. J Crohns Colitis. 10.1093/ecco-jcc/jjw189. PubMed
  136. Xu Z, et al. 2016. Nat Commun. 7:10728. PubMed
  137. Li Q, et al. 2017. Front Immunol. 0.797916667. PubMed
  138. Bekeschus S, et al. 2016. J Leukoc Biol. 100: 791 - 799. PubMed
  139. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  140. Gunasena M, et al. 2022. Sci Rep. 12:12377. PubMed
  141. Hornigold K, et al. 2022. Front Immunol. 13:888415. PubMed
  142. Yang B, et al. 2022. Bioengineered. 13:2685. PubMed
  143. Willis RA, et al. 2021. Curr Protoc. 1:e36. PubMed
  144. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  145. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  146. Liu X, et al. 2016. Sci Rep. 6:36722. PubMed
  147. Rio M, et al. 2012. J Immunol. 188:4885. PubMed
  148. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  149. Bajpai G, et al. 2018. Nat Med. 24:1234. PubMed
  150. Wilmes A, et al. 2017. Toxicol In Vitro. 10.1016/j.tiv.2017.07.023. PubMed
  151. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  152. Chen YF, et al. 2019. J Biomed Sci. 26:85. PubMed
  153. Rio M, et al. 2011. Transplantation. 92:1085. PubMed
  154. Dominguez CX, et al. 2020. Cancer Discov. 0.577777778. PubMed
  155. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  156. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  157. Xu Q, et al. 2021. Theranostics. 11:1937. PubMed
  158. Wen T, et al. 2022. Cancer Immunol Res. 10:162. PubMed
  159. Ma K, et al. 2022. iScience. 25:104347. PubMed
  160. Constant DA, et al. 2022. Immunohorizons. 6:416. PubMed
  161. Koyama T, et al. 2022. Curr Issues Mol Biol. 44:3146. PubMed
  162. Dong M, et al. 2022. J Immunother Cancer. 10:. PubMed
  163. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  164. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  165. Bruno MEC, et al. 2022. Geroscience. 44:1761. PubMed
  166. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
  167. Kakiyama G, et al. 2020. J Lipid Res. 61:1629. PubMed
  168. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  169. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  170. Yokota-Nakatsuma A, et al. 2016. Sci Rep. 6:37914. PubMed
  171. Kenna T, et al. 2010. J Immunol. 184:598. PubMed
  172. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  173. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  174. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  175. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  176. Woo MS, et al. 2021. J Exp Med. :218. PubMed
  177. Chen RH, et al. 2021. Front Immunol. 11:594775. PubMed
  178. Gerson-Gurwitz A, et al. 2021. Front Oncol. 11:766298. PubMed
  179. Koutník J, et al. 2022. Cell Commun Signal. 20:54. PubMed
  180. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  181. Cérbulo-Vázquez A, et al. 2022. PLoS One. 17:e0264566. PubMed
  182. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  183. Tatangelo V, et al. 2022. Front Oncol. 12:877495. PubMed
  184. Huang T, et al. 2022. Nat Commun. 13:3489. PubMed
  185. Kissel T, et al. 2022. Sci Adv. 8:eabm1759. PubMed
  186. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  187. Haunerdinger V, et al. 2021. Front Immunol. 12:740047. PubMed
  188. DiMuzio JM, et al. 2021. Vaccine X. 8:100098. PubMed
  189. Lei L, et al. 2021. Front Cell Dev Biol. 659744:9. PubMed
  190. Frafjord A, et al. 2020. Scand J Immunol. e12889:92. PubMed
  191. Lownik JC, et al. 2020. Biochem Biophys Rep. 24:100803. PubMed
  192. Labi V, et al. 2019. Genes Dev. 33:1673. PubMed
  193. Gensterblum E,et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.09.011. PubMed
  194. Zischke J, et al. 2017. PLoS Pathogens. 13(6):e1006454. PubMed
  195. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  196. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  197. Sun F, et al. 2022. Cell Death Dis. 13:181. PubMed
  198. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  199. Wang X, et al. 2022. Elife. 11:. PubMed
  200. Swarnalekha N, et al. 2021. Sci Immunol. 6:. PubMed
  201. Witt G, et al. 2020. Cell Biol Toxicol. . PubMed
  202. Marongiu L, et al. 2013. PLoS One. 8:75684. PubMed
  203. Nouraei N,et al. 2017. Exp Neurol.. 10.1016/j.expneurol.2017.10.017. PubMed
  204. Melcher M, et al. 2017. Stem Cell Res Ther . 10.1186/s13287-017-0601-7. PubMed
  205. Clancy-Thompson E, et al. 2015. Cancer Immunol Res. 3: 956-967. PubMed
  206. Wang L, et al. 2016. Oncogene. 10.1038/onc.2016.380. PubMed
  207. Zhang Z, et al. 2021. Front Immunol. 12:699478. PubMed
  208. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  209. Chen Y, et al. 2021. Braz J Med Biol Res. 54:e9570. PubMed
  210. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  211. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  212. Yoshikawa AM, et al. 2021. Nat Commun. 12:7106. PubMed
  213. Ikebuchi R, et al. 2016. Sci Rep. 6:35002. PubMed
  214. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  215. Nightingale K, et al. 2018. Cell Host Microbe. 24:447. PubMed

Antigen Details

Antigen References

1. Current Protocols in Immunology (John Wiley & Sons New York) Unit 6.24 Detection of Intracellular Cytokines by Flow Cytometry (Barbara Foster and Calman Prussin NIAID NIH Bethesda MD).
2. Sander B, et al. 1991. Immunol. Rev. 119:65.
3. Sander B, et al. 1993. J. Immunol. Meth. 166:201.
4. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.

Gene ID
NA
Go To Top Version: 4    Revision Date: 07/25/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account