PE anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
Ave. Rating
3 reviews
Product Citations
publications
390
C57BL/6 splenocytes stained with 390 PE
  • 390
    C57BL/6 splenocytes stained with 390 PE
See PE spectral data
Cat # Size Price Quantity Avail. Save
102407 50 µg $90
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102408 200 µg $250
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 102412).

Application References

(PubMed link indicates BioLegend citation)
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Wang L, et al. 2008. Cancer Res. 68:5639. PubMed
  2. Qayum N, et al. 2009. Cancer Res. 69:6347. PubMed
  3. Chong J, et al. 2010. J Biol Chem. 285:36179. PubMed
  4. Fokas E, et al. 2012. Cancer Res. 72:239. PubMed
  5. Reymond N, et al. 2012. J Cell Biol. 199:653. PubMed
  6. Baker M, et al. 2013. PLoS One. 8:62516. PubMed
  7. Aschermann S, et al. 2013. Proc Natl Acad Sci U S A. 110:19042. PubMed
  8. Im J, et al. 2012. Int J Cancer. 132:315. PubMed
  9. Moding E, et al. 2015. Sci Transl Med. 7:278. PubMed
  10. Groppa E, et al. 2015. EMBO Mol Med. 2015; 7: 1366 - 1384. PubMed
  11. Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
  12. Nakagawa A, et al. 2016. Sci Rep. 6: 25009. PubMed
  13. Padhye A, et al. 2019. Sci Rep. 9:4819. PubMed
  14. Lucotti S, et al. 2019. J Clin Invest. 130:1845. PubMed
  15. Dubey LK, et al. 2019. Cell Rep. 27:2442. PubMed
  16. Toyama K, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1392. PubMed
  17. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  18. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  19. Zhao T et al. 2018. Cell stem cell. 23(1):31-45 . PubMed
  20. Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed
  21. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  22. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  23. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  24. Hepler C et al. 2017. eLife. 6 pii: e27669. PubMed
  25. Santini MP, et al. 2020. Cell Rep. 30:555. PubMed
  26. Hou M, et al. 2020. Stem Cell Res Ther. 11:55. PubMed
RRID
AB_312902 (BioLegend Cat. No. 102407)
AB_312903 (BioLegend Cat. No. 102408)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/19/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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