Purified anti-human TNAP Antibody

Pricing & Availability
Clone
W8B2 (See other available formats)
Regulatory Status
RUO
Other Names
Tissue Non-specific Alkaline Phosphatase, mesenchymal stem cell antigen-1, MSC, W8B2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W8B2_Pure_020808
Human retinoblastoma cell line WERI-RB-1 stained with purified W8B2, followed by biotinylated anti-mouse IgG and Sav-PE
  • W8B2_Pure_020808
    Human retinoblastoma cell line WERI-RB-1 stained with purified W8B2, followed by biotinylated anti-mouse IgG and Sav-PE
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327302 100 µg $206
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Description

The W8B2 antibody reacts with an antigen, TNAP (Tissue non-specific alkaline phosphatase), that is selectively expressed on bone marrow mesenchymal stem cells, but not on placenta-derived mesenchymal stem cells. It was previously reported that the antibody recognized MSCA-1, mesenchymal stem cell antigen-1. Mesenchymal stem cells or MSCs were originally named as bone marrow stromal cells. They are a population of adult stem cells with a large capacity for self-renewal and multipotency for differentiation into a variety of cell types including osteoblasts, chondrocytes, myocytes, adipocytes, β-pancreatic islets cells and certain neuronal cells. MSCs reside in many tissues, such as bone marrow, placenta, adipose tissue, adult peripheral blood, fetal blood, skin, as well as liver and lung. Besides their plasticity for tissue repair, MSCs also exhibit a powerful immunosuppressive activity and play important role in supporting hematopoiesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Retinoblastoma cell line (Weri-RB-1 cells)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Battula VL, et al. 2009. Haematologica. 94:173.
Product Citations
  1. Tobys D, et al. 2020. Traffic. 22:6. PubMed
RRID
AB_940682 (BioLegend Cat. No. 327302)

Antigen Details

Distribution

Bone marrow mesenchymal stem cells

Cell Type
Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Battula VL, et al. 2007. Differentiation. 75:279.

Gene ID
249 View all products for this Gene ID
UniProt
View information about TNAP on UniProt.org
Go To Top Version: 2    Revision Date: 09/06/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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