Purified anti-Parkin Antibody

Pricing & Availability
Clone
A15165D (See other available formats)
Other Names
PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15165D_PURE_Parkin_Antibody_1_120219
Western blot of purified anti-Parkin antibody (clone A15165D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A15165D_PURE_Parkin_Antibody_1_120219
    Western blot of purified anti-Parkin antibody (clone A15165D). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A15165D_PURE_Parkin_Antibody_2_120219
    IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A15165D_PURE_Parkin_Antibody_3_120219
    IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A15165D_PURE_Parkin_Antibody_4_120219
    IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, Cat. No. 929701) and Boost DAB Enhancer (Cat. No. 926701) were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A15165D_PURE_Parkin_Antibody_5_120219
    IHC staining of purified anti-Parkin antibody (clone A15165D) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, Cat. No. 929701) and Boost DAB Enhancer (Cat. No. 926701) were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
870501 25 µg $90
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870502 100 µg $235
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Description

Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile parkinsonism (AR-JP).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length human Parkin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2820194 (BioLegend Cat. No. 870501)
AB_2820195 (BioLegend Cat. No. 870502)

Antigen Details

Structure
Human parkin is a 465 amino acid protein with a predicted molecular mass of ~52 kD.
Distribution

Tissue distribution: Brain, heart, testis, and skeletal muscle
Cellular distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum

Function
Parkin is involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
Interaction
Ubiquitin, PINK1
Ligand/Receptor
BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
Cell Type
Neurons
Biology Area
Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family
Ligases
Antigen References
  1. Tay SP, et al. 2010. J Biol Chem. 285:29231-8.
  2. Pawlyk AC, et al. 2003. J Biol Chem. 278:48120-8
Gene ID
5071 View all products for this Gene ID
UniProt
View information about Parkin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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