- Clone
- A15165E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A15165E_PURE_Parkin_Antibody_1_121819.png A15165E_PURE_Parkin_Antibody_1_121819.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A15165E_PURE_Parkin_Antibody_1_121819.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-Parkin antibody (clone A15165E) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Parkin antibody (clone A15165E) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Parkin antibody (clone A15165E) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, AEC, Cat. No. 929801) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Parkin antibody (clone A15165E) on formalin-fixed paraffin-embedded Parkinson’s disease substantia nigra tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, Cat. No. 929701) and Boost DAB Enhancer (Cat. No. 926701) were used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
870401 | 25 µg | $106 | ||||
870402 | 100 µg | $276 |
Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile parkinsonism (AR-JP).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full-length human Parkin protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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- RRID
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AB_2820192 (BioLegend Cat. No. 870401)
AB_2820193 (BioLegend Cat. No. 870402)
Antigen Details
- Structure
- Human Parkin is a 465 amino acid protein with a predicted molecular mass of ~52 kD.
- Distribution
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Tissue distribution: Brain, heart, testis, and skeletal muscle
Cellular distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum - Function
- Parkin is involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
- Interaction
- Ubiquitin, PINK1
- Ligand/Receptor
- BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
- Cell Type
- Neurons
- Biology Area
- Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
- Molecular Family
- Ligases
- Antigen References
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- Tay SP, et al. 2010. J Biol Chem. 285: 29231-8.
- Pawlyk AC, et al. 2003. J Biol Chem. 278(48):48120-8.
- Gene ID
- 5071 View all products for this Gene ID
- UniProt
- View information about Parkin on UniProt.org
Related FAQs
Other Formats
View All Parkin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Parkin | A15165E | IHC-P |
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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