- Clone
- MG1-45 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse (BALB/c) IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/MG1-45_GoChIP_IgG1_Iso-Con_060618_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-HDAC1 (Clone 10E2/HDAC1, Cat. No. 815103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG1, κ Isotype Control Antibody (Clone MG1-45, Cat. No. 401409) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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401401 | 50 µg | $47 | ||||
401402 | 500 µg | $129 |
The isotype of MG1-45 immunoglobulin is mouse IgG1, κ. It was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Trinitrophenol + KLH
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The immunoglobulin was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC, ICFC - Quality tested
ChIP - Verified
WB, IP, ICC, IHC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.
- Application Notes
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The MG1-45 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 401404) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 401408) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
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Application References
(PubMed link indicates BioLegend citation) - Product Citations
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- RRID
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AB_2801452 (BioLegend Cat. No. 401401)
AB_2801451 (BioLegend Cat. No. 401402)
Antigen Details
- Gene ID
- NA
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All ReagentsDescription | Clone | Applications |
---|---|---|
Purified Mouse IgG1, κ Isotype Ctrl | MG1-45 | FC,ICFC,ICC,IHC,IP,WB,ChIP |
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl | MG1-45 | FC,ICFC,WB,IP,ICC,IHC,FA |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified Mouse IgG1, κ Isotype Ctrl
Chromatin Immunoprecipitations (ChIP) were performed with cr... -
Ultra-LEAF™ Purified Mouse IgG1, κ Isotype Ctrl
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