Purified Streptavidin

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Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Cat # Size Price Quantity Avail. Save
280302 250 µg $35.00
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Technical data sheet

Product Details

Formulation
Phosphate-buffered solution containing 0.09% sodium azide.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ELISA Capture, ELISA Detection, IF, IHC-F, IHC-P, IP, WB - Reported in the literature

Recommended Usage

To coat one 96-well plate dilute 24 µl of Purified Streptavidin into 11.976ml of 1X Coating Buffer (Cat# 421701, Coating Buffer 5X concentrate). Final concentration in the coated plate is 2 µg/ml. This recommendation is to perform the protocol for HLA class I ELISA to evaluate Flex-T™ peptide exchange. For other applications the reagent needs to be optimized.

Antigen Details

Structure
52.8 kD tetrameric protein.
Distribution
Streptavidin binds to biotin with high affinity.
Ligand Receptor
Biotin.
Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID
NA

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