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RBC Lysis Buffer (10X)

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RUO
Other Names
Red blood cell lysis buffer
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420301 100 mL $65
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420302 500 mL $206
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Description

Red Blood Cell (RBC) Lysis Buffer has been designed, formulated, and tested to ensure optimal lysis of RBCs in single cell suspensions with minimal effects on leukocytes. RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. Nucleated RBCs are not effectively lysed with ammonium chloride. Test

Product Details
Technical data sheet

Product Details

Formulation
This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples.
Preparation
The pH of the 1X solution should fall within the range of pH 7.1-7.4. Adjust the pH if necessary. Warm the 1X solution to room temperature prior to use.
Storage & Handling
Store RBC lysis buffer between 2°C and 8°C.
Application Notes

A. Lysis of Mouse Spleen RBCs:
1. Harvest mouse spleen and prepare a single cell suspension.
2. Pellet the cells by centrifugation (350 x g); aspirate the supernatant.
3. Dilute the 10X Red Blood Cell Lysis Buffer to 1X working concentration with deionized water and resuspend the pellet in 5 ml of 1X Lysis Buffer.
4. Incubate on ice for 4-5 minutes with occasional shaking.
5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS.
6. Spin the cells (350 x g) and discard the supernatant.
7. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer Cat. No. 420201), wash 1X.
8. Count cells, adjust density, and proceed with cell staining procedures.
B. Lysis of Human Peripheral Blood RBCs:
1. Dilute the 10X RBC Lysis Buffer to 1X working concentration with deionized water. Warm the 1X solution to room temperature prior to use.
2. Add 2.0 ml of 1X RBC Lysis Buffer to each tube containing up to 100 µl of whole blood.
3. Gently vortex each tube immediately after adding the lysing solution. Incubate at room temperature, protected from light, for 10-15 minutes.
4. Centrifuge 350 x g for 5 minutes. Aspirate supernatant without disturbing pellet.
5. Resuspend the pellet in the appropriate buffer (e.g., BioLegend Cell Staining Buffer, Cat. No. 420201), wash 1X.
6. Resuspend and proceed with further procedures.

Application References

(PubMed link indicates BioLegend citation)
  1. Adams RA, et al. 2007. J. Exp. Med. 204:571.
  2. Feild-Corbett C, et al. 2009. Stem Cells. PubMed
  3. de Resende MM, et al. 2010. Physiol Genomics. 42:437. PubMed
  4. Roybal JD, et al. 2011. Mol Cancer Res. 9:25. PubMed
  5. Boldin MP, et al. 2011. J Exp Med. PubMed
  6. Kahlenberg JM, et al. 2011. J. Immunol. 187:6143. PubMed
  7. Deiuliis JA, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:399. PubMed
  8. Wu CY, et al. 2012. PLoS One. 7:e42365. PubMed
  9. Hsia BJ, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  10. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  11. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. PubMed
  12. Saraiva AM, 2013. Hum Immunol. 74:207. PubMed
  13. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed
  14. Ma Dy, et al. 2013. J Virol. 87:3361. PubMed
  15. Han W, 2013. J. Immunol. 190:4786. PubMed
  16. Garcia-Acros I, et al. 2013. J Biol Chem. 288:14046. PubMed
  17. Zhao JL, et al. 2013. Elife. 21:537. PubMed
  18. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. PubMed
  19. Yamasaki S, et al. 2013. Clin Vaccine immunol. 20:1508. PubMed
  20. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  21. Liu X, et al. 2013. Invest Ophthalmol Vis Sci. 54:7386. PubMed
  22. Fullerton AM, et al. 2013. Toxicol Sci. 136:72. PubMed
  23. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  24. Varga T, et al. 2013. J. Immunol. 191:5695. PubMed
  25. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  26. Chang YJ, et al. 2014. Cytotherapy. 16:560. PubMed
  27. Kim MJ, et al. 2014. Mol Pharmacol. 86:284. PubMed
  28. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  29. Szulzewsky F, et al. 2015. PloS One. 10:116644. PubMed
Product Citations
  1. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  2. Strickley JD, et al. 2019. Nature. 575:519. PubMed
  3. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  4. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  5. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  6. Deiuliis J, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 302:L399. PubMed
  7. Jin J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2901. . PubMed
  8. Barros-Martins J, et al. 2016. J Immunol. 196: 3642 - 3652. PubMed
  9. Zhou B, et al. 2020. Nat Immunol. 1.868055556. PubMed
  10. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  11. Asem M, et al. 2020. Cancer Res. 1156:80. PubMed
  12. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  13. Tsai AG, et al. 2020. Nat Med. 408:26. PubMed
  14. Herisson F, et al. 2018. Nat Neurosci. 21:1209. PubMed
  15. Pracht K, et al. 2021. Eur J Immunol. 51:1089. PubMed
  16. Radwanska M, et al. 2020. Methods Mol Biol. 2116:721. PubMed
  17. Rendon CJ, et al. 2022. Front Endocrinol (Lausanne). 13:995499. PubMed
  18. Effern M, et al. 2022. STAR Protoc. 3:101038. PubMed
  19. Della Verde G, et al. 2022. Life Sci Alliance. 5:. PubMed
  20. Su C, et al. 2021. Front Oncol. 11:719564. PubMed
  21. Jacoby E, et al. 2021. NPJ Regen Med. 6:58. PubMed
  22. Chen Z, et al. 2020. Glia. 68:2148. PubMed
  23. Seow JJW, et al. 2021. Front Med (Lausanne). 8:603374. PubMed
  24. Shin J, et al. 2020. Hum Gene Ther. . PubMed
  25. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  26. Spengler J, et al. 2016. J Infect Dis. 214: S308 - S318. PubMed
  27. Zheng M, et al. 2016. J Immunol. 196: 4122 - 4131. PubMed
  28. St-Pierre S, et al. 2016. PLoS One. 11: 0150230. PubMed
  29. Ensan S, et al. 2015. Nat Immunol. 17: 159-168. PubMed
  30. Jaiswal M, et al. 2015. Sci Rep. 5: 15221. PubMed
  31. Hou J, et al. 2015. J Mol Cell Biol. 7: 338-350. PubMed
  32. Fujii H, et al. 2015. PLoS One. 10: 0133216. PubMed
  33. Liu W, et al. 2017. Int J Environ Res Public Health. 10.3390/ijerph14060592. PubMed
  34. Pietrocola F, et al. 2017. Autophagy. 0.935416667. PubMed
  35. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  36. Wang J, et al. 2020. Sci Rep. 10:12785. PubMed
  37. Zhang J, et al. 2021. Aging (Albany NY). 13:17880. PubMed
  38. Press AT, et al. 2021. EMBO Mol Med. 13:e14436. PubMed
  39. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  40. Ma H, et al. 2021. Front Immunol. 12:760095. PubMed
  41. McGill CJ, et al. 2021. STAR Protoc. 2:100948. PubMed
  42. Lutze J, et al. 2022. Methods Mol Biol. 2394:299. PubMed
  43. Hosseini A, et al. 2022. Evid Based Complement Alternat Med. 2022:5639226. PubMed
  44. Bikorimana JP, et al. 2022. Cell Rep Med. 3:100534. PubMed
  45. Zhang L, et al. 2022. JCI Insight. 7:. PubMed
  46. Baldominos P, et al. 2022. STAR Protoc. 3:101795. PubMed
  47. Ma Y, et al. 2014. Cancer Res. 74:436. PubMed
  48. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  49. Moraes-Vieira P, et al. 2016. Diabetes. 65: 1317 - 1327. PubMed
  50. Garcia-Fabiani MB, et al. 2020. STAR Protoc. :1. PubMed
  51. Boldin M, et al. 2011. J Exp Med. 208:1189. PubMed
  52. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  53. Bravo–San Pedro JM, et al. 2019. Cell Metab. 30:754. PubMed
  54. Clark EL, et al. 2017. PLoS Genet. 13:e1006997. PubMed
  55. Cantor JR, et al. 2017. Cell. 169:258. PubMed
  56. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  57. Chen J, et al. 2022. Nat Commun. 13:6759. PubMed
  58. Wu Y, et al. 2021. Nat Biomed Eng. 5:1336. PubMed
  59. Meng S, et al. 2022. Exp Anim. . PubMed
  60. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  61. Johnson CL, et al. 2021. Am J Physiol Heart Circ Physiol. 321:H756. PubMed
  62. Stringhini M, et al. 2021. MAbs. 13:1868066. PubMed
  63. Devalaraja S, et al. 2020. Cell. 1098:180. PubMed
  64. Elisabeth Schrumpf, Xiaojun Jiang 2017. Physiol Rep. 10.14814/phy2.13117. PubMed
  65. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  66. Brunmeir R, et al. 2016. PLoS Genet. 12:e1006474. PubMed
  67. Pridans C, et al. 2016. J Immunol. 197: 2297 - 2305. PubMed
  68. Mircheff A, et al. 2016. Ocul Surf. 14:460-483. PubMed
  69. Pennington L, et al. 2016. Nat Commun. 7:11610. PubMed
  70. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  71. Sakamoto S, et al. 2020. OncoImmunology. 8(12):e1671760.. PubMed
  72. Zhai Z, et al. 2021. Medicine (Baltimore). 100:e26214. PubMed
  73. Hu D, et al. 2021. J Cell Mol Med. 25:2900. PubMed
  74. Jones N, et al. 2021. Nat Commun. 12:1209. PubMed
  75. Wang D, et al. 2021. Signal Transduct Target Ther. 6:376. PubMed
  76. Machado H, et al. 2021. PLoS Pathog. 17:e1009933. PubMed
  77. Yan X, et al. 2022. Int J Biol Sci. 18:585. PubMed
  78. Singh SS, et al. 2021. Eur J Med Chem. 216:113312. PubMed
  79. Pokhrel RH, et al. 2022. Int J Mol Sci. 23:. PubMed
  80. Rico C, et al. 2012. Carcinogenesis. 33:2283. PubMed
  81. Hamour S, et al. 2015. PLoS One. 10: 0136238. PubMed
  82. Bazett M, Haston M 2016. Sci Rep. 6:19189. PubMed
  83. Luo B, et al. 2016. J Neurosci. 36: 9590 - 9603. PubMed
  84. Patera A, et al. 2016. J Leukoc Biol. 100(6):1239-1254. PubMed
  85. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  86. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  87. Jiang J, et al. 2018. Cancer Med. 3.504861111. PubMed
  88. Chen Z, et al. 2017. Cancer Res. 77:2266. PubMed
  89. Kim M, et al. 2017. Int J Mol Sci. 10.3390/ijms18061275. PubMed
  90. Mo L, et al. 2022. Front Oncol. 12:871489. PubMed
  91. Huang L, et al. 2021. J Cell Physiol. 236:7308. PubMed
  92. Whytock KL, et al. 2022. iScience. 25:104772. PubMed
  93. Neufeld L, et al. 2021. Sci Adv. 7:. PubMed
  94. Friedman G, et al. 2020. Nat Cancer. 1:692. PubMed
  95. Kumar A, et al. 2021. Part Fibre Toxicol. 18:43. PubMed
  96. Tanaka Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  97. Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed
  98. Jeong J, et al. 2021. Immune Netw. 21:e31. PubMed
  99. Sitaraman S, et al. 2021. JCI Insight. 6:. PubMed
  100. Orr AN, et al. 2021. Int J Mol Sci. 22:. PubMed
  101. Facchini M, et al. 2014. J Vis Exp. 85: 51019. PubMed
  102. Ilievski V, et al. 2015. PLoS One. 10: 0136502. PubMed
  103. Saraiva A, et al. 2013. Hum Immunol. 74:207. PubMed
  104. Wang K, et al. 2017. Molecular Immunology. 10.1016/j.molimm.2017.04.016. PubMed
  105. Appelbe O, et al. 2017. J Control Release.. 10.1016/j.jconrel.2017.09.043. PubMed
  106. Tang J, et al. 2017. Proc Natl Acad Sci U S A. 114:E7441. PubMed
  107. Liccardi G, et al. 2019. Mol Cell. 73:413. PubMed
  108. Patil R, et al. 2019. J Biomed Opt. 24:1. PubMed
  109. Ongaro T, et al. 2020. Oncotarget. 3.026388889. PubMed
  110. Kahlenberg J, et al. 2011. J Immunol. 187:6143. PubMed
  111. Kildisiute G, et al. 2021. Sci Adv. 7: . PubMed
  112. Schiferle EB, et al. 2021. Sci Adv. 7: . PubMed
  113. Devalaraja S, et al. 2020. STAR Protoc. 1:100188. PubMed
  114. Bigenwald C, et al. 2021. Nat Med. 27:851. PubMed
  115. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  116. Dang Y, et al. 2022. Cancer Cell Int. 22:88. PubMed
  117. Deng J, et al. 2021. Breast Cancer Res Treat. 185:85. PubMed
  118. Vivanco Gonzalez N, et al. 2022. STAR Protoc. 3:101280. PubMed
  119. Qin L, et al. 2022. Nat Commun. 13:6051. PubMed
  120. Escudero-Lara A, et al. 2022. Front Behav Neurosci. 16:946975. PubMed
  121. Han W, et al. 2013. J Immunol. 190:4786. PubMed
  122. Yamasaki S, et al. 2013. Clin Vaccine Immunol. 20:1508. PubMed
  123. Fukui R, et al. 2016. J Immunol. 196: 416 - 427. PubMed
  124. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  125. Martínez-Muriana A, et al. 2020. Stem Cell Res Ther. 11:53. PubMed
  126. Pennig J, et al. 2019. Sci Rep. 9:17937. PubMed
  127. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  128. Anstine L,et al. 2017. J Am Heart Assoc.. 10.1161/JAHA.117.007097. PubMed
  129. Zhang L, et al. 2021. Methods Mol Biol. 2388:175. PubMed
  130. Evans AK, et al. 2020. Neurobiol Dis. 146:105089. PubMed
  131. Judge SJ, et al. 2020. J Clin Invest. 130:3051. PubMed
  132. Choi JW, et al. 2020. PLoS One. 15:e0235518. PubMed
  133. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  134. Jiang S, et al. 2017. Toxicology Lett. 10.1016/j.toxlet.2017.03.015. PubMed
  135. Agrawal V, et al. 2016. J Leukoc Biol. 100: 261 - 274. PubMed
  136. Elphinstone R, et al. 2017. Infect Immun. 10.1128/IAI.00371-17. PubMed
  137. Kernfeld EM, et al. 2018. Immunity. 48:1258. PubMed
  138. Nwani NG, et al. 2019. Cancers (Basel). 0.806944444. PubMed
  139. Ramalingam P, et al. 2020. Nat Commun. 11:666. PubMed
  140. Yu Z, et al. 2021. Front Oncol. 11:659251. PubMed
  141. Deng J, et al. 2021. Nat Cancer. 2:503. PubMed
  142. Beaudoin-Bussières G, et al. 2022. Cell Rep. 38:110368. PubMed
  143. Hu H, et al. 2021. Cancer Immunol Res. 9:1298. PubMed
  144. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  145. So EY, et al. 2021. Am J Physiol Cell Physiol. 321:C569. PubMed
  146. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  147. Garcia-Arcos I, et al. 2013. J Biol Chem. 288:14046. PubMed
  148. Sci T 2013. Toxicol Sci. 136:72. PubMed
  149. Metz H, et al. 2016. Proc Natl Acad Sci U S A. 113: 8795 - 8800. PubMed
  150. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  151. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  152. Alghamri MS, et al. 2020. Methods Enzymol. 632:215. PubMed
  153. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  154. Rosenbluth JM, et al. 2020. Nat Commun. 11:1711. PubMed
  155. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  156. Ullah I, et al. 2021. Immunity. 54:2143. PubMed
  157. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  158. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  159. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  160. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  161. Zhu QY, et al. 2021. Nat Commun. 12:6624. PubMed
  162. Patel M, et al. 2021. Cancer Res. 81:3985. PubMed
  163. Shi X, et al. 2021. Nat Commun. 12:2547. PubMed
  164. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  165. Lizotte P, et al. 2016. Sci Rep. 6:31745. PubMed
  166. Wen J, et al. 2017. J Hematol Oncol. 10.1186/s13045-017-0489-9. PubMed
  167. Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed
  168. Hang J, et al. 2019. Cancer Med. 1.254166667. PubMed
  169. Tan X, et al. 2019. Sci Rep. 2.7125. PubMed
  170. Lienemann PS, et al. 2020. Adv Sci (Weinh). 7:1903395. PubMed
  171. Hills LB, et al. 2021. J Immunol. 206:89. PubMed
  172. Page N, et al. 2021. Nat Commun. 12:1009. PubMed
  173. Pace J, et al. 2022. J Biomed Opt. 27:. PubMed
  174. Pedicona F, et al. 2022. Sci Signal. 15:eabl7989. PubMed
  175. Fidalgo MF, et al. 2022. Life Sci Alliance. 5:. PubMed
  176. Stirling ER, et al. 2022. J Immunother Cancer. 10:. PubMed
  177. Zhao J, et al. 2013. Elife. 21:537. PubMed
  178. Weber G, et al. 2015. Surgery. 158: 248-254. PubMed
  179. Jiang H, et al. 2016. Nat Med. 10.1038/nm.4123. PubMed
  180. Elgueta R, et al. 2016. J Immunol. 197: 3970 - 3981. PubMed
  181. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  182. Schweppe RE, et al. 2019. Mol Cancer Res. 17:1036. PubMed
  183. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  184. Yang A, et al. 2018. Cancer Discov. 0.525. PubMed
  185. Rentsendorj A, et al. 2017. Brain Behav Immun. 10.1016/j.bbi.2017.08.019. PubMed
  186. Yan Q, et al. 2022. Clin Transl Med. 12:e1084. PubMed
  187. Zhai K, et al. 2021. Nat Cancer. 2:1136. PubMed
  188. Zhao D, et al. 2020. Cancer Discov. 10:1374. PubMed
  189. Castoldi F, et al. 2020. Cell Death Discov. 0.339583333. PubMed
  190. Wang L, et al. 2020. EMBO J. 39:e104514. PubMed
  191. Morris C, et al. 2017. MBio. 10.1128/mBio.00036-17. PubMed
  192. Theurl I, et al. 2016. Nat Med. 10.1038/nm.4146. PubMed
  193. Dyck S, et al. 2018. J Neuroinflammation. 0.6875. PubMed
  194. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  195. Gangwar RS, et al. 2020. Sci Rep. 10:14348. PubMed
  196. Hiza H, et al. 2021. Sci Rep. 11:13190. PubMed
  197. Ito T, et al. 2021. Nat Commun. 12:2232. PubMed
  198. Hollerbach A, et al. 2021. J Thromb Haemost. . PubMed
  199. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  200. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  201. Zhang Y, et al. 2022. Vaccines (Basel). 10: . PubMed
  202. Majumdar D, et al. 2021. Curr Protoc. 1:e216. PubMed
  203. Michelotti TC, et al. 2022. Front Cell Dev Biol. 10:1025240. PubMed
  204. Ren X, et al. 2022. STAR Protoc. 3:101818. PubMed
  205. Varga T, et al. 2013. J Immunol. 191:5695. PubMed
  206. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  207. Tsao P, et al. 2016. Proc Natl Acad Sci U S A. 113: 8242 - 8247. PubMed
  208. Soncini M, et al. 2016. Proc Natl Acad Sci U S A. 113: E6219 - E6227. PubMed
  209. Yang C, et al. 2016. Sci Rep. 6:34643. PubMed
  210. Nowyhed H, et al. 2017. Sci Rep. 7:40273. PubMed
  211. De Domenico E, et al. 2020. STAR Protoc. 1:100233. PubMed
  212. Wang Z, et al. 2020. Cell Rep. 33:108472. PubMed
  213. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  214. Gladow N, et al. 2020. PLoS One. 15:e0227734. PubMed
  215. Ramachandran P, et al. 2019. Nature. 575:512. PubMed
  216. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  217. Bowers J, et al. 2017. Front Immunol. 10.3389/fimmu.2017.01221. PubMed
  218. Feng D, et al. 2022. Nat Commun. 13:5554. PubMed
  219. Saber M, et al. 2021. J Neurosci Res. 99:1136. PubMed
  220. Xiang Y, et al. 2022. Skelet Muscle. 12:19. PubMed
  221. Yamazaki T, et al. 2021. Oncoimmunology. 10:1962592. PubMed
  222. Zhang J, et al. 2020. Pain. 161:1381. PubMed
  223. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  224. Canali S, et al. 2017. Blood. 129(4):405-414. PubMed
  225. Shen X, et al. 2016. Sci Rep. 6:38778. PubMed
  226. Sauter K, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G533 - G547. PubMed
  227. Song C, et al. 2015. Blood. 126: 1813 - 1822. PubMed
  228. Sasaki Y, et al. 2015. PLoS One. 10: 0131176. PubMed
  229. Ansari M, et al. 2017. Cell Signal. 10.1016/j.cellsig.2017.04.014. PubMed
  230. Ahmad S, et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. 10.1016/j.pnpbp.2017.06.034. PubMed
  231. Bichsel C, et al. 2017. Sci Rep. 10.1038/s41598-017-09928-1. PubMed
  232. James M, et al. 2017. J Nucl Med. . 10.2967/jnumed.117.189597. PubMed
  233. Annibaldi A, et al. 2018. Mol Cell. 69:566. PubMed
  234. Hill SJ, et al. 2018. Cancer Discov. 1.308333333. PubMed
  235. Tulasi DY, et al. 2021. Cell Mol Gastroenterol Hepatol. 11:1437. PubMed
  236. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  237. Xue X, et al. 2021. Cent Eur J Immunol. 46:191. PubMed
  238. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  239. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  240. Ma D, et al. 2013. J Virol. 87:3361. PubMed
  241. Kraft P, et al. 2013. Stroke. 44:3202. PubMed
  242. Kim M, et al. 2014. Mol Pharmacol. 86:284. PubMed
  243. Cekic C, et al. 2014. Cancer Res. 74:7250. PubMed
  244. Pankova D, et al. 2016. Mol Cancer Res. 14: 287 - 295. PubMed
  245. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  246. Resende M, et al. 2010. Physiol Genomics. 42:437. PubMed
  247. McLaughlin S, et al. 2019. Nat Commun. 3.795833333. PubMed
  248. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  249. Zhang Z, et al. 2017. Journal of Translational Medicine. 10.1186/s12967-017-1196-6. PubMed
  250. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  251. Soileau AM, et al. 2022. Vet Sci. 9:. PubMed
  252. McClellan BL, et al. 2022. STAR Protoc. 3:101357. PubMed
  253. Governa V, et al. 2022. Proc Natl Acad Sci U S A. 119. PubMed
  254. Deng Y, et al. 2021. Nat Commun. 12:7041. PubMed
  255. Mai CL, et al. 2021. Theranostics. 11:1059. PubMed
  256. Chen P, et al. 2021. Clin Transl Med. 11:e462. PubMed
  257. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  258. Linder A, et al. 2020. EMBO J. 39:e105071. PubMed
  259. Muñoz-Ruiz M, et al. 2016. Nat Immunol. 10.1038/ni.3424. PubMed
  260. Chang Y, et al. 2014. Cytotherapy. 16:560. PubMed
  261. Hsia B, et al. 2012. J Allergy Clin Immunol. 130:205. PubMed
  262. Mochizuki M,et al. 2017. J Am Heart Assoc. . 10.1161/JAHA.117.005920. PubMed
  263. Roh–Johnson M, et al. 2017. Dev Cell. 43:549. PubMed
  264. Müller C, et al. 2018. Elife. 7:e34985. PubMed
  265. Cordo' V, et al. 2022. Nat Commun. 13:1048. PubMed
  266. Hallisey M, et al. 2022. STAR Protoc. 3:101163. PubMed
  267. Denoth-Lippuner A, et al. 2021. Cell Stem Cell. 28:2020. PubMed
  268. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  269. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  270. Sobecki M, et al. 2022. Cell Stem Cell. 29:1459. PubMed
  271. Guo W, et al. 2013. J Gerontol A Biol Sci Med Sci. . PubMed
  272. Liu Y, et al. 2015. Sci Rep. 5: 15134. PubMed
  273. Hodson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 2039-2046. PubMed
  274. Munson D, et al. 2016. Proc Natl Acad Sci U S A. 113: 8272 - 8277. PubMed
  275. Ubellacker J, et al. 2017. Breast Cancer Res. 10.1186/s13058-017-0815-8. PubMed
  276. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  277. Trial J, et al. 2020. Geroscience. . PubMed
  278. Denning W, et al. 2011. PLoS One. 6:e16897. PubMed
  279. Weaver J, et al. 2017. Part Fibre Toxicol. 10.1186/s12989-017-0206-4. PubMed
  280. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  281. Wong C, et al. 2017. J Immunol. 10.4049/jimmunol.1700397. PubMed
  282. Visweshwaran SP, et al. 2022. Front Cell Dev Biol. 10:1008898. PubMed
  283. Li Q, et al. 2022. Signal Transduct Target Ther. 7:222. PubMed
  284. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  285. Vechetti IJ, et al. 2021. FASEB J. 35:e21644. PubMed
  286. Saber M, et al. 2020. Eur J Neurosci. 52:2791. PubMed
  287. Wu R, et al. 2020. J Immunother Cancer. 8:00. PubMed
  288. Yu H, et al. 2016. Sci Rep. 6:35190. PubMed
  289. Now H, Yoo J 2016. Cell Mol Immunol. 10.1038/cmi.2016.32. PubMed
  290. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  291. YC S, SJ L 2015. Sci Rep. 5: 12578. PubMed
  292. Szulzewsky F, et al. 2015. PLoS One. 10:116644. PubMed
  293. Wu C, et al. 2012. PLoS One. 7:e42363. PubMed
  294. Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
  295. Roybal J, et al. 2011. Mol Cancer Res. 9:25. PubMed
  296. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  297. Park HJ, et al. 2021. Int J Mol Sci. 22:. PubMed
  298. Cebrian-Silla A, et al. 2021. Elife. 10:. PubMed
  299. Yeini E, et al. 2021. Nat Commun. 12:1912. PubMed
  300. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  301. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  302. Kucykowicz S, et al. 2022. STAR Protoc. 3:101356. PubMed
  303. Kim TW, et al. 2022. Mol Ther Oncolytics. 26:1. PubMed
  304. Kathania M, et al. 2022. Cell Rep. 40:111345. PubMed
  305. Shaashua L, et al. 2022. Nat Commun. 13:6513. PubMed
  306. Cekic C, et al. 2013. J Exp Med. 210:2693. PubMed
  307. Field-Corbett C, et al. 2009. Stem Cells. 27:662. PubMed
  308. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed
  309. Zheng W, et al. 2019. Cell Rep. 29:1747. PubMed
  310. Tomas C, et al. 2019. Sci Rep. 9:11464. PubMed
  311. Kim JK, et al. 2019. Bioengineering (Basel). 6:24. PubMed
  312. Alizadeh A, et al. 2018. J Neuroinflammation. 15:53. PubMed
  313. Guo P, et al. 2017. J Clin Invest. 127:4242. PubMed
  314. Tsai SC, et al. 2017. Biomicrofluidics. 11:034122. PubMed
  315. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  316. Alam A, et al. 2022. STAR Protoc. 3:101563. PubMed
  317. Xiang Y, et al. 2022. Exp Mol Med. 54:1011. PubMed
  318. Guo Z, et al. 2022. Neurobiol Pain. 12:100096. PubMed
  319. Guo P, et al. 2022. Nat Cell Biol. 24:99. PubMed
  320. Wahane S, et al. 2021. Sci Adv. 7:. PubMed
  321. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  322. Huang N, et al. 2021. Nat Med. 27:892. PubMed
  323. Song X, et al. 2021. FEBS Open Bio. 11:2453. PubMed
  324. Chen S, et al. 2020. Cancer Immunol Res. 1064:8. PubMed
  325. Erady C, et al. 2021. NPJ Genom Med. 6:04. PubMed
  326. de Silva S, et al. 2020. Cancer Immunol Res. 0.493055556. PubMed
  327. Wan C, et al. 2021. Cancer Res. 81:158. PubMed
  328. Wang X, et al. 2016. J Virol. 90: 10247 - 10258. PubMed
  329. Mohammed R, et al. 2016. Cell Rep. 14:760-771. PubMed
  330. Durward-Diioia M, et al. 2015. Infect Immun . 83: 4759 - 4771. PubMed
  331. Birnie K, et al. 2015. Mol Cancer Res. 13: 1106 - 1118. PubMed
  332. Liu X, et al. 2013. Invest Ophthalmol Vis Sci . 54:7386. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA
Go To Top Version: 3    Revision Date: 06/10/2022

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