Alexa Fluor® 700 anti-human CD64 Antibody

Pricing & Availability
Clone
S18012E (See other available formats)
Regulatory Status
RUO
Other Names
FcγRI, FcR I
Isotype
Mouse IgG1, κ
S18012E_A700_CD64_Antibody_020322
Human peripheral blood monocytes were stained with anti-human CD64 (clone S18012E) Alexa Fluor® 700 (filled histogram), or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • S18012E_A700_CD64_Antibody_020322
    Human peripheral blood monocytes were stained with anti-human CD64 (clone S18012E) Alexa Fluor® 700 (filled histogram), or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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399611 25 tests $124.00
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399612 100 tests $265.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD64-Fc (1257-FC-050)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

In-house testing suggests that S18012E partially blocks the binding of clone 10.1 but not clone S18012C. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB). 

RRID
AB_2910460 (BioLegend Cat. No. 399611)
AB_2910460 (BioLegend Cat. No. 399612)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG receptor
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Hulett MD & Hogarth PM. 1994. Adv Immunol. 57:1-127. 
  2. van de Winkel JG & Capel PJ. 1993. Immunol Today. 14:215-21. 
Gene ID
2209 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 1    Revision Date: 02/03/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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