PE anti-human CD64 Antibody

Pricing & Availability
Clone
S18012E (See other available formats)
Regulatory Status
RUO
Other Names
FcγRI, FcR I
Isotype
Mouse IgG1, κ
S18012E_PE_CD64_Antibody_033120
Human peripheral blood monocytes were stained with CD64 (clone S18012E) PE (filled histogram) or mouse IgG1, κ (clone MOPC-21) PE isotype control (open histogram).
  • S18012E_PE_CD64_Antibody_033120
    Human peripheral blood monocytes were stained with CD64 (clone S18012E) PE (filled histogram) or mouse IgG1, κ (clone MOPC-21) PE isotype control (open histogram).
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Cat # Size Price Quantity Check Availability
399603 25 tests $110.00
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399604 100 tests $244.00
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcR I. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD64-Fc (1257-FC-050)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

In-house testing suggests that S18012E partially blocks the binding of clone 10.1 but not clone S18012C. Clone 10.1 recognizes the EC3 epitope of CD64. While both CD64 subunits contain EC3 domains, clone 10.1 preferentially binds to CD64A (FcγRIA) but not CD64B (FcγRIB). 

RRID
AB_2861009 (BioLegend Cat. No. 399603)
AB_2861010 (BioLegend Cat. No. 399604)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, dendritic cells, activated granulocytes

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG receptor
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Hulett MD & Hogarth PM. 1994. Adv Immunol. 57:1-127. 
  2. van de Winkel JG & Capel PJ. 1993. Immunol Today. 14:215-21. 
Gene ID
2209 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org
Go To Top Version: 1    Revision Date: 03/31/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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