Pacific Blue™ anti-mouse CD45.2 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

B10.S mouse thymocytes and splenocytes

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Violet Laser (405 nm)

Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation⁴, in vivo and in vitro blocking of B cell responses^1,2, and immunohistochemical staining of acetone-fixed frozen sections³. 

Application References

(PubMed link indicates BioLegend citation)

1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
6. Pascal V, et al. 2007. J. Immunol. 179:1751.
7. Burman AC, et al. 2007. Blood 110:1064.
8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
9. Phan TG, et al. 2007. Nature Immunol. 8:992.
10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed

Product Citations

1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
3. Collin R, et al. 2020. J Immunol. 205:133. PubMed
4. MacLean AJ, et al. 2022. Immunity. 55:718. PubMed
5. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
6. Vukovic N, et al. 2022. J Biol Chem. 298:102153. PubMed
7. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
8. Lu Z, et al. 2022. Cell Rep. 41:111482. PubMed
9. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
10. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
11. Valladao A, et al. 2016. J Immunol. 197(12):4541-4551. PubMed
12. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
13. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
14. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
15. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
16. Israelow B, et al. 2020. bioRxiv. . PubMed
17. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
18. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
19. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
20. Pascal V, et al. 2007. J Immunol. 179:1751. PubMed
21. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
22. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
23. Silva D, et al. 2011. Diabetes. 60:2102. PubMed
24. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
25. Yamamoto R et al. 2018. Cell stem cell. 22(4):600-607 . PubMed
26. Yalamanoglu A, et al. 2021. Front Immunol. 627944:12. PubMed
27. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
28. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
29. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
30. Wong J, et al. 2015. Elife. 3: 07839. PubMed
31. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
32. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
33. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
34. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
35. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
36. Keller CW, et al. 2020. Autophagy. 1:. PubMed
37. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
38. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
39. Lin G, et al. 2013. PLoS One. 8:e56014. PubMed
40. Zhu Y, et al. 2015. Neurobiol Dis. 74:114. PubMed
41. Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
42. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
43. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
44. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
45. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
46. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
47. Arsiwala T, et al. 2022. Int J Mol Sci. 23:. PubMed
48. Goldman E, et al. 2021. Cells. 10:. PubMed
49. Flommersfeld S, et al. 2021. Immunity. :. PubMed
50. Aegerter H, et al. 2020. Nat Immunol. 0.975694444. PubMed
51. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
52. Chen X, et al. 2019. Nat Commun. 4.421527778. PubMed
53. Sá da Bandeira D, et al. 2022. Cell Rep. 40:111114. PubMed
54. Dewas C, et al. 2015. J Immunol. 194:1372. PubMed
55. Kincaid E, et al. 2007. J Immunol. 179:3187. PubMed
56. Patel S, et al. 2019. Viruses. 0.877083333. PubMed
57. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
58. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
59. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
60. Pfefferlé M, et al. 2021. Front Immunol. 12:680855. PubMed
61. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
62. Cohen M, et al. 2014. EMBO J. 33:2906. PubMed
63. Ma K, et al. 2022. iScience. 25:104347. PubMed
64. Scholz F, et al. 2015. J Immunol. 195: 2335-2342. PubMed
65. Yabas M, et al. 2016. PLoS One. 11: 0146774. PubMed
66. Reddy Y 2009. Proc Natl Acad Sci U S A. 106:21689. PubMed
67. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
68. Udden SN et al. 2019. eLife. 8 pii: e40396. PubMed
69. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
70. Zeis P, et al. 2020. Immunity. 53:775. PubMed
71. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
72. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
73. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
74. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
75. Tullett KM, et al. 2020. Elife. 9:00. PubMed
76. Wang E, et al. 2013. Proc Natl Acad Sci U S A. 110:3901. PubMed
77. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
78. Müller A, et al. 2012. Immunity. 37:147. PubMed
79. Kratky W, et al. 2011. Proc Natl Acad Sci U S A. 108:17414. PubMed
80. Zlotoff D, et al. 2010. Blood. 115:1897. PubMed
81. Teijeira á, et al. 2020. Immunity. 52(5):856-871. PubMed
82. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
83. Nagalingam G, et al. 2017. J Immunol. 10.4049/jimmunol.1602069. PubMed
84. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
85. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
86. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
87. Vannini N, et al. 2016. Nat Commun. 7:13125. PubMed
88. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
89. Knier B, et al. 2018. Nat Immunol. 1.722916667. PubMed
90. Astuti Y, et al. 2017. Stem Cell Reports. 8(1):177-190. PubMed
91. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
92. Teijaro J, et al. 2014. Proc Natl Acad Sci U S A. 111:3799. PubMed

RRID

AB_492873 (BioLegend Cat. No. 109819)
AB_492872 (BioLegend Cat. No. 109820)

Antigen Details

Structure

Protein tyrosine phosphatase (PTP) family, 180-240 kD

Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function

Phosphatase, T and B cell activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers

Molecular Family

CD Molecules

Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID

19264 View all products for this Gene ID

UniProt

View information about CD45.2 on UniProt.org

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Other Formats